Transcription by T7 RNA polymerase of DNA containing abasic sites
| Autor: | Giselle Sanchez, Margaret D. Mamet-Bratley |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Apurinic Acid DNA Repair Transcription Genetic Epidemiology Health Toxicology and Mutagenesis Termination factor RNA-dependent RNA polymerase Biology Viral Proteins chemistry.chemical_compound Transcription (biology) Bacteriophage T7 RNA polymerase Escherichia coli medicine RNA polymerase I T7 RNA polymerase AP site Promoter Regions Genetic Genetics (clinical) RNA DNA-Directed RNA Polymerases Templates Genetic Molecular biology RNA Bacterial Biochemistry chemistry Oxidation-Reduction DNA Damage Plasmids medicine.drug |
| Zdroj: | Environmental and Molecular Mutagenesis. 23:32-36 |
| ISSN: | 1098-2280 0893-6692 |
| DOI: | 10.1002/em.2850230106 |
| Popis: | The effects of abasic (AP) sites on RNA synthesis were studied in vitro, using T7 RNA polymerase and a plasmid template containing a T7 promoter. The presence of increasing numbers of AP sites caused a progressive decline in RNA synthesis. The average RNA chain length, calculated from the ratio of initiation to chain elongation, decreased with increasing numbers of AP sites, revealing that complete blocks must occur during synthesis. The probability that RNA polymerase would be blocked at an AP site in the DNA template strand was estimated to be 0.3 in our experimental conditions. These results demonstrate that RNA synthesis by T7 RNA polymerase is inhibited by AP sites and that readthrough of the lesion occurs more frequently than premature chain termination. Chemical reduction of AP sites in the template did not change the block/bypass pattern. © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text