Extracellular calcium sensing in rat aortic vascular smooth muscle cells
| Autor: | Jacob Tfelt-Hansen, Sanela Smajilovic, Tue E H Christoffersen, Ernest F. Terwilliger, Søren P. Sheikh, Stig Haunsø, Ewa Lewin, Edward M. Brown, Jakob Lerche Hansen |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Cell type Vascular smooth muscle MAP Kinase Signaling System MAP Kinase Kinase 1 Biophysics chemistry.chemical_element Biology Calcium Biochemistry Muscle Smooth Vascular Internal medicine Extracellular medicine Animals RNA Messenger Rats Wistar Extracellular Signal-Regulated MAP Kinases Inositol phosphate Receptor Molecular Biology Aorta Cells Cultured Cell Proliferation chemistry.chemical_classification Cell growth DNA Cell Biology Rats Cell biology Endocrinology chemistry Protein Biosynthesis Calcium-sensing receptor Receptors Calcium-Sensing |
| Zdroj: | Biochemical and Biophysical Research Communications. 348:1215-1223 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.07.192 |
| Popis: | Extracellular calcium ( Ca 2 + o ) can act as a first messenger in many cell types through a G protein-coupled receptor, calcium-sensing receptor (CaR). It is still debated whether the CaR is expressed in vascular smooth muscle cells (VSMCs). Here, we report the expression of CaR mRNA and protein in rat aortic VSMCs and show that Ca 2 + o stimulates proliferation of the cells. The effects of Ca 2 + o were attenuated by pre-treatment with MAPK kinase 1 (MEK1) inhibitor, as well as an allosteric modulator, NPS 2390. Furthermore, stimulation of the VSMCs with Ca 2 + o -induced phosphorylation of ERK1/2, but surprisingly did not cause inositol phosphate accumulation. We were not able to conclusively state that the CaR mediates Ca 2 + o -induced cell proliferation. Rather, an additional calcium-sensing mechanism may exist. Our findings may be of importance with regard to atherosclerosis, an inflammatory disease characterized by abnormal proliferation of VSMCs and high local levels of calcium. |
| Databáze: | OpenAIRE |
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