Habitual high-protein diet does not influence muscle protein synthesis in response to acute resistance exercise in rats
| Autor: | Hideo Yoshizato, Satoru Ato, Yuki Maruyama, Riki Ogasawara |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Endocrinology Diabetes and Metabolism Protein metabolism Muscle Proteins 030209 endocrinology & metabolism Stimulation High-protein diet mTORC1 medicine.disease_cause Rats Sprague-Dawley 03 medical and health sciences Basal (phylogenetics) chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Humans Muscle Skeletal Muscle protein 030109 nutrition & dietetics Nutrition and Dietetics business.industry Resistance training Skeletal muscle Resistance Training Rats Endocrinology medicine.anatomical_structure chemistry Diet High-Protein Dietary Proteins business |
| Zdroj: | Nutrition. 78:110795 |
| ISSN: | 0899-9007 |
| DOI: | 10.1016/j.nut.2020.110795 |
| Popis: | Objectives: Resistance training combined with consumption of a high-protein diet (HPD) is typically recommended to increase muscle mass, as both acute resistance exercise (RE) and dietary protein intake stimulate mechanistic target of rapamycin complex 1 (mTORC1) and muscle protein synthesis (MPS). However, the effect of chronic HPD consumption on MPS response to an acute RE remains to be determined. Methods Male Sprague-Dawley rats aged 10 wk were fed HPD (50 kcal % protein, for 4 wk) or normal protein diet (NPD; 20 kcal % protein). After the 4-wk dietary intervention, the rats were fasted overnight and the right gastrocnemius muscle was subjected to percutaneous electrical stimulation to mimic acute RE, whereas the left gastrocnemius muscle served as control. The rats were sacrificed 6 h after exercise and the tissues were sampled immediately. Results The HPD group showed significantly lower fat mass and higher skeletal muscle mass than the NPD group without affecting body weight. Resting mTORC1 activity did not differ between the groups. Additionally, resting MPS was also unchanged after HPD. Acute RE significantly increased mTORC1 activity and MPS in both groups. However, differences in diet did not influence the response of mTORC1 activation to acute RE. Furthermore, HPD did not affect the response of MPS to acute RE. Conclusion The present results suggested that although 4 wk of HPD reduces body fat and increases skeletal muscle mass, it does not affect muscle protein synthesis at basal state, and in response to acute RE. |
| Databáze: | OpenAIRE |
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