Cultured human epidermis cells produce cell-associated interleukin 1-like prostaglandin E2- and collagenase-stimulating factors
| Autor: | Conrad Hauser, Jean-H. Saurat, Stephan Sizonenko, Fabienne Jaunin, Jean M. Dayer |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Adult
Lysis Adolescent medicine.medical_treatment Cell Biology Dinoprostone medicine Humans Prostaglandin E2 Child Molecular Biology Cells Cultured Skin integumentary system Epidermis (botany) Prostaglandins E Interleukin Cell Biology Fibroblasts Molecular biology Stimulation Chemical Kinetics Microbial Collagenase medicine.anatomical_structure Biochemistry Cell culture Collagenase Interleukin-1 medicine.drug Prostaglandin E |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 846:350-355 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(85)90005-9 |
| Popis: | In order to identify factors which may regulate the functions of dermal fibroblasts, cell lysates and conditioned media of cultured human epidermal cells were tested on dermal fibroblasts for the stimulation of prostaglandin E2- and collagenase-production. Both prostaglandin E2- and collagenase-stimulating activities appeared during epidermal cell culture: after 2 d they were detected in the cell lysate, and after 4 d of culture they were found also in the conditioned media. Molecular sieving chromatography of epidermal cell lysates led to the detection of two main peaks showing concomitant prostaglandin E2- and collagenase-stimulating activities at Mr approximately equal to 18 000 and Mr approximately equal to 10 000. A single peak of concomitant prostaglandin E2- and collagenase-stimulating activities were seen at Mr approximately equal to 10 000 in the epidermal cell conditioned media. This suggests that the cell-associated concomitant prostaglandin E2- and collagenase-stimulating activities are processed from a common precursor molecule and released. Collagenase-stimulating activity without accompanying prostaglandin E2 was also detected in the range of Mr approximately equal to 30 000-45 000. |
| Databáze: | OpenAIRE |
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