Human cardiac fibroblasts produce pro-inflammatory cytokines upon TLRs and RLRs stimulation
Autor: | Tuan T Nguyen, Zhe Li, Alan Valaperti |
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Přispěvatelé: | University of Zurich, Valaperti, Alan |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Clinical Biochemistry Cell 610 Medicine & health Inflammation Stimulation 1308 Clinical Biochemistry 030204 cardiovascular system & hematology Article Proinflammatory cytokine 1307 Cell Biology 03 medical and health sciences 0302 clinical medicine Human Cardiac Fibroblasts 1312 Molecular Biology medicine Humans Myofibroblasts Receptor Molecular Biology Cells Cultured business.industry Interleukins Toll-Like Receptors Cell Biology General Medicine Immunity Innate Cell biology TLR2 030104 developmental biology medicine.anatomical_structure Cell culture Receptors Pattern Recognition TLR3 10033 Clinic for Immunology Cytokines Inflammation Mediators medicine.symptom business |
Zdroj: | Molecular and Cellular Biochemistry |
ISSN: | 1573-4919 0300-8177 |
DOI: | 10.1007/s11010-021-04157-7 |
Popis: | Heart inflammation is one of the major causes of heart damage that leads to dilated cardiomyopathy and often progresses to end-stage heart failure. In the present study, we aimed to assess whether human cardiac cells could release immune mediators upon stimulation of Toll-like receptors (TLRs) and Retinoic acid-inducible gene (RIG)-I-like receptors (RLRs).Commercially available human cardiac fibroblasts and an immortalized human cardiomyocyte cell line were stimulated in vitro with TLR2, TLR3, and TLR4 agonists. In addition, cytosolic RLRs were activated in cardiac cells after transfection of polyinosinic-polycytidylic acid (PolyIC). Upon stimulation of TLR3, TLR4, MDA5, and RIG-I, but not upon stimulation of TLR2, human cardiac fibroblasts produced high amounts of the pro-inflammatory cytokines IL-6 and IL-8. On the contrary, the immortalized human cardiomyocyte cell line was unresponsive to the tested TLRs agonists. Upon RLRs stimulation, cardiac fibroblasts, and to a lesser extent the cardiomyocyte cell line, induced anti-viral IFN-β expression.These data demonstrate that human cardiac fibroblasts and an immortalized human cardiomyocyte cell line differently respond to various TLRs and RLRs ligands. In particular, human cardiac fibroblasts were able to induce pro-inflammatory and anti-viral cytokines on their own. These aspects will contribute to better understand the immunological function of the different cell populations that make up the cardiac tissue. |
Databáze: | OpenAIRE |
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