Immunization of dogs with a DNA vaccine induces protection against rabies virus
| Autor: | Alvaro Aguilar-Setién, Yves Jacob, Emmanuel Desmezieres, M. Aubert, Pierre Perrin, E. Loza-Rubio, Florence Cliquet, Noël Tordo, Corinne Jallet |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Blotting Western medicine.disease_cause Antibodies Viral DNA vaccination Dogs medicine Vaccines DNA Animals Mononegavirales Lyssavirus Duck embryo vaccine General Veterinary General Immunology and Microbiology biology Rabies virus Public Health Environmental and Occupational Health Rhabdoviridae biology.organism_classification medicine.disease Virology Vaccination Infectious Diseases Rabies Vaccines Molecular Medicine Rabies Female Immunization |
| Zdroj: | Vaccine. 18(5-6) |
| ISSN: | 0264-410X |
| Popis: | Rabies is a fatal encephalomyelitis which is transmitted to man, mostly by dogs in developing countries. This zoonosis can be prevented by vaccination of humans before or after exposure. However, a more radical approach is possible, involving the elimination of the principal vector/reservoir by vaccinating dogs. The vaccine must be effective, safe and inexpensive. Mass production of plasmids is possible and DNA-based immunization with a plasmid encoding the antigen responsible for inducing protection seems to be more cost-effective than classical techniques involving cell culture. Beagles were immunized by intramuscular (i.m.) injection with a plasmid encoding the rabies virus (PV strain) glycoprotein. Neutralizing antibodies against both wild-type rabies virus and European Bat Lyssaviruses (EBL1 and EBL2) were detected after a single injection and a boost, but levels of neutralizing antibodies against EBL1 were low. Moreover, all vaccinated dogs were protected against a lethal challenge with a wild-type dog rabies strain. This is one of the first studies to demonstrate that dogs can be protected by DNA vaccines, and opens important perspectives for rabies control. |
| Databáze: | OpenAIRE |
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