Unique Response of Double-Stranded Oligonucleotides Containing a Single 8-Oxo-7,8-Dihydroguanine to Gamma Rays in the Frozen Aqueous State at 77 K
| Autor: | Mikinori Kuwabara, Yoshiharu Iida, Osamu Inanami, Eiko Ohtsuka, Hideo Inoue |
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| Rok vydání: | 2000 |
| Předmět: |
Gel electrophoresis
Guanine Radiation Aqueous solution Oligonucleotide Stereochemistry Oligonucleotides Biophysics Water Ionizing radiation Cold Temperature chemistry.chemical_compound Piperidines chemistry Biochemistry Gamma Rays Humans Electrophoresis Polyacrylamide Gel Radiology Nuclear Medicine and imaging Irradiation Polyacrylamide gel electrophoresis Cytosine DNA Damage |
| Zdroj: | Radiation Research. 154:582-589 |
| ISSN: | 1938-5404 0033-7587 |
| Popis: | Iida, Y., Inanami, O., Inoue, H., Ohtsuka, E. and Kuwabara, M. Unique Response of Double-Stranded Oligonucleotides Containing a Single 8-Oxo-7,8-Dihydroguanine to Gamma Rays in the Frozen Aqueous State at 77 K. Two kinds of double-stranded oligonucleotides containing a single 8-oxo-7,8-dihydroguanine were labeled with 32P at their 5′ ends and exposed to γ rays in the frozen aqueous state at 77 K, where both direct and quasi-direct effects of ionizing radiation predominate. Analysis of the oligonucleotides with 20% denaturing polyacrylamide gel electrophoresis revealed no difference in the immediate induction of strand breaks between oligonucleotides containing 8-oxo-7,8-dihydroguanine and their corresponding oligonucleotides with normal guanine, but piperidine-sensitive damage was induced more frequently in the former than in the latter. Sequence analysis of irradiated oligonucleotides showed that not only 8-oxo-7,8-dihydroguanine but also its neighboring bases and the cytosine residue that is pa... |
| Databáze: | OpenAIRE |
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