Structural, functional properties and immunomodulatory activity of isolated Inca peanut (Plukenetia volubilis L.) seed albumin fraction
| Autor: | Zhuo Jiang, Li Pan, Wen Jiamin, Fengying Lin, Xiaowei Ma, Bing Du |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Arachis Nitric Oxide Biochemistry 03 medical and health sciences Mice 0404 agricultural biotechnology Differential scanning calorimetry Structural Biology Albumins Animals Immunologic Factors Denaturation (biochemistry) Secretion Food science Lymphocytes Molecular Biology Protein secondary structure Cell Proliferation chemistry.chemical_classification Mice Inbred BALB C 030109 nutrition & dietetics biology Chemistry Albumin Water 04 agricultural and veterinary sciences General Medicine Hydrogen Peroxide biology.organism_classification 040401 food science Molecular Weight Enzyme RAW 264.7 Cells Solubility Seeds Tumor necrosis factor alpha Muramidase Plukenetia volubilis |
| Zdroj: | International journal of biological macromolecules. 118 |
| ISSN: | 1879-0003 |
| Popis: | This work aimed to determine the structural, functional properties and immunomodulatory activity of an albumin fraction isolated from Inca peanut seed (AF-IPS). Structural characterization revealed that AF-IPS contains two polypeptides with molecular weight ranges of 25–45 and 10–15 kDa. AF-IPS is mainly composed of α-helix, β-sheet, and β-turn secondary structures. Differential scanning calorimetry analysis indicated that the denaturation temperature of AF-IPS at room temperature is 101.93 °C. AF-IPS possessed excellent protein solubility (63.0%), water holding capacity (1.59 g/g), foaming (350.2%) and emulsifying (13.0 mL/g) ability. Heat treatment improved protein solubility, oil holding capacity, and foaming and emulsifying ability. AF-IPS exhibited immunomodulatory activity by stimulating the proliferation and enhancing the TNF-α secretion of splenic lymphocytes, and through increasing the cellular lysosomal enzyme and pinocytic activities, and by moderately promoting the NO and H2O2 production of RAW264.7 cells. Our findings indicated that AF-IPS has potential applications in functional and pharmaceuticals foods. |
| Databáze: | OpenAIRE |
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