Fluctuations in haemocyte density and microbial load may be used as indicators of fungal pathogenicity in larvae of Galleria mellonella

Autor:	David A. Bergin, Kevin Kavanagh, Marc Brennan
Rok vydání:	2003
Předmět:	Larva animal structures Hemocytes genetic structures biology Virulence Inoculation fungi Immunology Fungi imperfecti Moths biology.organism_classification Microbiology Yeast Galleria mellonella Infectious Diseases parasitic diseases Candida albicans Animals human activities Bacteria Pyralidae
Zdroj:	Microbes and infection. 5(15)
ISSN:	1286-4579
Popis:	A positive correlation exists between the pathogenicity of bacteria and fungi when evaluated in the insect Galleria mellonella and mice. This work sought to determine whether fluctuations in the number of haemocytes and the proliferation of yeast cells in infected larvae could be used to determine the relative pathogenicity of a range of yeast isolates. Larvae were inoculated with 1 x 10(6) stationary-phase yeast cells and incubated in the dark at 30 degrees C for 48 h. The results indicated that larvae inoculated with the most pathogenic isolates (i.e. those capable of killing >80% of infected larvae) showed a significant reduction in haemocyte density. Larvae inoculated with isolates of low pathogenicity (i.e. capable of killing
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae370579dea0eeecb6fe41e11b6b693c https://pubmed.ncbi.nlm.nih.gov/14670452 Zobrazit plný text záznamu Full Text from ScienceDirect