Phenotype of NK cells and cytotoxic/apoptotic mediators expression in ectopic pregnancy

Autor: Herman Haller, Petar Vukelić, Daniel Rukavina, Arnela Redzovic, Danijela Veljković, Gordana Laškarin, Tamara Gulic
Rok vydání: 2010
Předmět:
Antigens
Differentiation
T-Lymphocyte
Cytotoxicity
Immunologic
Ectopic pregnancy
BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences. Immunology
Apoptosis
NK cells
TNF-Related Apoptosis-Inducing Ligand
Interleukin 21
Pregnancy
Immunology and Allergy
Cytotoxic T cell
Granulysin
reproductive and urinary physiology
Interleukin-15
education.field_of_study
Decidua
Obstetrics and Gynecology
NK receptors
Flow Cytometry
Immunohistochemistry
Killer Cells
Natural
medicine.anatomical_structure
Interleukin 15
embryonic structures
Cytokines
BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti. Imunologija
Female
Pregnancy
Tubal
cytotoxic mediators
ectopic pregnancy
interleukin-15
medicine.medical_specialty
animal structures
Fas Ligand Protein
Immunology
Population
Biology
Statistics
Nonparametric
Immunophenotyping
Andrology
Antigens
CD
Internal medicine
medicine
Humans
education
urogenital system
Perforin
BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Ginekologija i opstetricija
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Gynecology and Obstetrics
Trophoblast
Endocrinology
Reproductive Medicine
biology.protein
Zdroj: American Journal of Reproductive Immunology
Volume 64
ISSN: 1600-0897
1046-7408
DOI: 10.1111/j.1600-0897.2010.00844.x
Popis: Phenotype of NK cells and cytotoxic/apoptotic mediators expression in ectopic pregnancy. Am J Reprod Immunol 2010 Problem The expression of cytotoxic/apoptotic mediators and the phenotype characteristics of uterine NK cells (uNK) in tubal ectopic pregnancy (EP) were investigated. Method of study Samples of uterine decidua and tubal mucosa as well as peripheral blood (PB) of the same women with EP were used for phenotype characterization of NK cells and detection of cytotoxic/apoptotic mediators and IL-15. Results In tubal mucosa, perforin, FasL, granulysin and IL-15 were almost completely absent, but they were present in normal and EP uterine deciduas. TRAIL was present on trophoblast and tubal mucosa, contrary to its lack in normal and EP uterine decidua. CD16-CD56dim NK cells, mostly CD94- and NKG2A-, predominate in tubal mucosa, whereas CD16-CD56bright NK cells, predominantly CD94+ and NKG2A+ prevail in EP uterine decidua. NK cells at the EP implantation site express lower percentages of perforin and granulysin, but they express a higher percentage of TRAIL than do EP uterine decidual and PB NK cells. Lower percentage of TNF-α-expressing and IL-4-expressing NK cells were found at the implantation site compared to EP uterine decidua. Conclusions Authentic uNK cell population seems to be insufficient to restrict trophoblast invasion because of low expression of cytotoxic/apoptotic mediators.
Databáze: OpenAIRE
Externí odkaz: