Riboflavin deficiency affects lipid metabolism partly by reducing apolipoprotein B100 synthesis in rats
Autor: | Bian Xiangyu, Changjiang Guo, Zhanxin Yao, Bailin Li, Weina Gao, Wang Yawen, Qingao Xu |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty Apolipoprotein B Riboflavin Endocrinology Diabetes and Metabolism Cholesterol VLDL Clinical Biochemistry Protein Disulfide-Isomerases Biochemistry 03 medical and health sciences chemistry.chemical_compound Riboflavin Deficiency Internal medicine medicine Animals heterocyclic compounds RNA Messenger Rats Wistar Protein disulfide-isomerase Molecular Biology Triglycerides Membrane Glycoproteins 030109 nutrition & dietetics Nutrition and Dietetics biology Triglyceride Cholesterol Endoplasmic reticulum digestive oral and skin physiology food and beverages Lipid metabolism Cholesterol LDL Metabolism Lipid Metabolism Rats Oxidative Stress 030104 developmental biology Endocrinology Liver chemistry Apolipoprotein B-100 biology.protein lipids (amino acids peptides and proteins) |
Zdroj: | The Journal of Nutritional Biochemistry. 70:75-81 |
ISSN: | 0955-2863 |
Popis: | Lipid metabolism is dependent on riboflavin status. Apolipoprotein B100 plays an important role in lipids transportation. This study was aimed to investigate the effect of riboflavin status on lipid metabolism and explore its association with apolipoprotein B100 synthesis in vivo. Riboflavin deficiency was developed in rats by feeding riboflavin-deficient diets. Compared to the control rats, the mRNA and protein expressions of apolipoprotein B100 were significantly reduced in riboflavin-deficient rats. Endoplasmic reticulum oxidoreductin 1 (ERO1) and protein disulfide isomerase (PDI), two enzymes involved in the oxidative folding of apolipoprotein B100, were also lowered remarkably in expression at protein level. Meanwhile, total cholesterol and triglyceride levels were decreased in the plasma and increased in the liver of riboflavin-deficient rats. The plasma very low-density lipoprotein cholesterol (VLDL-c) and low-density lipoprotein cholesterol (LDL-c) were also reduced in riboflavin-deficient rats. Our findings demonstrate that riboflavin deficiency affects lipid metabolism partly by reducing apolipoprotein B100 synthesis. |
Databáze: | OpenAIRE |
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