Riboflavin deficiency affects lipid metabolism partly by reducing apolipoprotein B100 synthesis in rats

Autor: Bian Xiangyu, Changjiang Guo, Zhanxin Yao, Bailin Li, Weina Gao, Wang Yawen, Qingao Xu
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Apolipoprotein B
Riboflavin
Endocrinology
Diabetes and Metabolism
Cholesterol
VLDL
Clinical Biochemistry
Protein Disulfide-Isomerases
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Riboflavin Deficiency
Internal medicine
medicine
Animals
heterocyclic compounds
RNA
Messenger
Rats
Wistar
Protein disulfide-isomerase
Molecular Biology
Triglycerides
Membrane Glycoproteins
030109 nutrition & dietetics
Nutrition and Dietetics
biology
Triglyceride
Cholesterol
Endoplasmic reticulum
digestive
oral
and skin physiology
food and beverages
Lipid metabolism
Cholesterol
LDL
Metabolism
Lipid Metabolism
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
Liver
chemistry
Apolipoprotein B-100
biology.protein
lipids (amino acids
peptides
and proteins)
Zdroj: The Journal of Nutritional Biochemistry. 70:75-81
ISSN: 0955-2863
Popis: Lipid metabolism is dependent on riboflavin status. Apolipoprotein B100 plays an important role in lipids transportation. This study was aimed to investigate the effect of riboflavin status on lipid metabolism and explore its association with apolipoprotein B100 synthesis in vivo. Riboflavin deficiency was developed in rats by feeding riboflavin-deficient diets. Compared to the control rats, the mRNA and protein expressions of apolipoprotein B100 were significantly reduced in riboflavin-deficient rats. Endoplasmic reticulum oxidoreductin 1 (ERO1) and protein disulfide isomerase (PDI), two enzymes involved in the oxidative folding of apolipoprotein B100, were also lowered remarkably in expression at protein level. Meanwhile, total cholesterol and triglyceride levels were decreased in the plasma and increased in the liver of riboflavin-deficient rats. The plasma very low-density lipoprotein cholesterol (VLDL-c) and low-density lipoprotein cholesterol (LDL-c) were also reduced in riboflavin-deficient rats. Our findings demonstrate that riboflavin deficiency affects lipid metabolism partly by reducing apolipoprotein B100 synthesis.
Databáze: OpenAIRE
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