Stromal cell‐derived factor‐1 regulates the secretion of interleukin‐1β in the temporomandibular joint of rats with synovial inflammation
| Autor: | Dong Qi, Jingjing Kong, Qiang Wang, Shuzhen Sun, Yan Chen, Ronglin Wang, Liang Han, Ping Ji, Yujun Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Cancer Research medicine.medical_specialty Bodily Secretions Stromal cell Interleukin-1beta IL‐1β Inflammation Pathology and Forensic Medicine Proinflammatory cytokine AMD3100 Pathogenesis 03 medical and health sciences 0302 clinical medicine Synovitis Internal medicine occlusal interference model medicine Animals Stromal cell-derived factor 1 Rats Wistar CXCR4 biology Temporomandibular Joint business.industry Synovial Membrane Interleukin Original Articles medicine.disease SDF‐1 Chemokine CXCL12 Temporomandibular joint Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Otorhinolaryngology 030220 oncology & carcinogenesis biology.protein Periodontics Original Article Oral Surgery medicine.symptom Stromal Cells business synovitis |
| Zdroj: | Journal of Oral Pathology & Medicine |
| ISSN: | 1600-0714 0904-2512 |
| Popis: | Background Synovitis is characterized by the infiltration of inflammatory cells and often accompanies the pathological progression of the clinical symptoms affecting the temporomandibular joint (TMJ), such as pain, snapping, and limited mouth opening. It has been suggested that the signal transduction pathway and resultant proinflammatory mediators play important roles in the pathogenesis of synovitis. Therefore, in this present research, we aimed to investigate the changes in the expressions of stromal cell‐derived factor 1 (SDF‐1) and interleukin (IL)‐1β in rats with occlusal interference. Materials and Methods We divided 36 male Wistar rats into the following groups: Group A (control group), Group B (occlusal interference group), and Group C (AMD3100 group). Synovial inflammation was induced in the rats in Groups B and C to establish the occlusal interference model. The inflammatory changes were detected, and the expressions of SDF‐1 and IL‐1β in the synovium were assayed via immunostaining and a real‐time quantitative polymerase chain reaction (PCR). Results In Group B, obvious inflammatory changes were observed in the synovial membranes; additionally, the SDF‐1 and IL‐1β expression levels were significantly higher at the protein and mRNA levels. However, in Group C, these experimental results were inhibited by an injection with AMD3100. Conclusion These results may indicate that SDF‐1 regulates the expression level of inflammatory factors, such as IL‐1β, in the synovial membranes of rats with occlusal interference. Our findings suggest that the SDF‐1 axis may contribute to the onset of synovitis during the development of TMJ joint disease. |
| Databáze: | OpenAIRE |
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