Fluid shear stress increases transepithelial transport of Ca2+ in ciliated distal convoluted and connecting tubule cells
| Autor: | René J. M. Bindels, Sami G. Mohammed, Femke Latta, Joost G. J. Hoenderop, Ronald Roepman, Francisco J. Arjona |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Kidney Reabsorption Chemistry Cilium Stimulation Nephron Biochemistry Connecting tubule 03 medical and health sciences Transient receptor potential channel Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] 030104 developmental biology medicine.anatomical_structure Endocrinology Internal medicine Paracellular transport Genetics medicine Biophysics Molecular Biology Biotechnology |
| Zdroj: | The Faseb Journal, 31, 1796-1806 The Faseb Journal, 31, 5, pp. 1796-1806 |
| ISSN: | 0892-6638 |
| DOI: | 10.1096/fj.201600687RRR |
| Popis: | Contains fulltext : 173023.pdf (Publisher’s version ) (Closed access) In kidney, transcellular transport of Ca2+ is mediated by transient receptor potential vanilloid 5 and Na+-Ca2+ exchanger 1 proteins in distal convoluted and connecting tubules (DCT and CNT, respectively). It is not yet understood how DCT/CNT cells can adapt to differences in tubular flow rate and, consequently, Ca2+ load. This study aims to elucidate the molecular mechanisms by which DCT/CNT cells sense fluid dynamics to control transepithelial Ca2+ reabsorption and whether their primary cilia play an active role in this process. Mouse primary DCT/CNT cultures were subjected to a physiologic fluid shear stress (FSS) of 0.12 dyn/cm2 Transient receptor potential vanilloid 5 and Na+-Ca2+ exchanger 1 mRNA levels were significantly increased upon FSS exposure compared with static controls. Functional studies with 45Ca2+ demonstrated a significant stimulation of transepithelial Ca2+ transport under FSS compared with static conditions. Primary cilia removal decreased Ca2+ transport in both static and FSS conditions, a finding that correlated with decreased expression of genes involved in transepithelial Ca2+ transport; however, FSS-induced stimulation of Ca2+ transport was still observed. These results indicate that nephron DCT and CNT segments translate FSS into a physiologic response that implicates an increased Ca2+ reabsorption. Moreover, primary cilia influence transepithelial Ca2+ transport in DCT/CNT, yet this process is not distinctly coupled to FSS sensing by these organelles.-Mohammed, S. G., Arjona, F. J., Latta, F., Bindels, R. J. M., Roepman, R., Hoenderop, J. G. J. Fluid shear stress increases transepithelial transport of Ca2+ in ciliated distal convoluted and connecting tubule cells. |
| Databáze: | OpenAIRE |
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