Proteomic analysis of plasma from patients with systemic lupus erythematosus: Increased presence of haptoglobin α2 polypeptide chains over the α1 isoforms
| Autor: | Antonio Lario, Salvador A Arias, Francisco Navarro-Pelayo, Norberto Ortego-Centeno, Montserrat Carrascal, Victoria Longobardo, Jaime Sancho, Ricardo Sola, Mercedes Zubiaur, José-Luis Callejas-Rubio, Joaquín Abián, Enrique Raya-Alvarez, Esther J. Pavón, Pilar M. Muñoz, Ana B. Martín |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Biochemistry Gene Frequency Internal medicine medicine Humans Lupus Erythematosus Systemic Protein Isoforms Electrophoresis Gel Two-Dimensional skin and connective tissue diseases Molecular Biology Allele frequency Autoimmune disease Lupus erythematosus Haptoglobins biology Haptoglobin Case-control study Hypergammaglobulinemia medicine.disease Phenotype Endocrinology Case-Control Studies Immunology biology.protein Biomarkers Systemic vasculitis |
| Zdroj: | PROTEOMICS. 6:S282-S292 |
| ISSN: | 1615-9853 |
| DOI: | 10.1002/pmic.200500404 |
| Popis: | In the present study plasma samples from 15 systemic lupus erythematosus (SLE) patients and 16 healthy controls of initially unknown haptoglobin (Hp) phenotype were separated by 2-DE, and tryptic digests of the excised Hpalpha polypeptide chain spots were analyzed by MALDI-TOF-MS. Selected tryptic peptides were sequenced by nano-(n)ESI-IT MS/MS. The six major Hp phenotypes were present, although with distinct frequencies in controls and SLE patients. Thus, there were an increased proportion of SLE patients with Hp 2-2, or Hp 2-1S phenotypes. The Hp phenotype distribution resulted in allele frequencies of 0 625 (Hp(2)), 0.281 (Hp(1S)), and 0.093 (Hp(1F)) in healthy controls, correlating fairly well with the allele frequencies of European populations. In contrast, the Hp allele frequencies of the SLE patients were 0.733 (Hp(2)), 0.233 (Hp(1S)), and 0.033 (Hp1(1F)), which clearly indicated an increased frequency of Hp(2), a similar proportion of Hp(1S) and a diminished proportion of Hp(1F) in SLE patients compared with that in healthy controls. Preferential Hpalpha2 expression in SLE patients may contribute to some of the clinical manifestations of the disease such as hypergammaglobulinemia, systemic vasculitis, and cardiovascular disorders. |
| Databáze: | OpenAIRE |
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