Antinociceptive and anti-inflammatory effect of Poincianella pyramidalis (Tul.) L.P. Queiroz
| Autor: | Brancilene Santos de Araujo, Geraldo Célio Brandão, Fabiolla Rocha Santos Passos, Marlange Almeida Oliveira Melo, Jullyana S.S. Quintans, Sabrina Zelice da Cruz de Moraes, Emiliano Barreto, Ariel de Souza Graça, Charles dos Santos Estevam, Juliane Pereira da Silva, Andrea Yu Kwan Villar Shan, Lucindo José Quintans Júnior |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Antioxidant medicine.drug_class DPPH medicine.medical_treatment Ethyl acetate Anti-Inflammatory Agents Acetates Carrageenan Anti-inflammatory Antioxidants 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Phenols In vivo Drug Discovery medicine Animals Gallic acid 030304 developmental biology Pain Measurement Pharmacology 0303 health sciences Analgesics Traditional medicine Dose-Response Relationship Drug Plant Extracts Fabaceae chemistry 030220 oncology & carcinogenesis visual_art visual_art.visual_art_medium Plant Bark Bark |
| Zdroj: | Journal of ethnopharmacology. 254 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacology relevance Poncianella pyramidalis (Leguminosae) is a Caatinga plant used in folk medicine because of its pharmacological properties, which include anti-inflammatory action. However, chemical compounds responsible for this effect have not yet been identified. Aim of the study This study aimed to evaluate the antioxidant, antinociceptive and anti-inflammatory effects of the ethyl acetate fraction from the inner bark of P. pyramidalis. Material and methods Total phenol content (TP) was estimated using the Folin-Ciocalteu reagent, while in vitro antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Chemical identification was done using LC-PDA/MS and LC-ESI/MS/MS. In vivo antinociceptive and anti-inflammatory properties were investigated using formalin, mechanical hypernociception and carrageenan-induced pleurisy assays in mice. Results TP was 525.08 ± 17.49 μg mg−1 gallic acid equivalent. The ethyl acetate fraction (EAF) inhibited 87.76% of the DPPH radical with an EC50 of 22.94 μg mL−1 and Antioxidant Activity Index of 1.74. LC-PDA/MS and LC-ESI/MS/MS identified 15 compounds that are mostly derived from gallic and ellagic acids. Regarding in vivo antinociceptive and anti-inflammatory activity, EAF (100 mg kg-1) significantly reduced the nociceptive response in the second phase of the formalin assay by 50% (p Conclusion These results demonstrate EAF from the inner bark of P. pyramidalis has strong in vitro antioxidant effect as well as in vivo antinociceptive and anti-inflammatory activities, which may be attributed to the bark being rich in phenolic compounds derived from gallic acid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect