Prevention of the development of melphalan resistance in vitro by selenite
| Autor: | Gerald D. Frenkel, Paula B. Caffrey, Ming Zhu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Melphalan
Endocrinology Diabetes and Metabolism Clinical Biochemistry chemistry.chemical_element Drug resistance Biology Pharmacology Biochemistry Inorganic Chemistry Ovarian tumor chemistry.chemical_compound Sodium Selenite immune system diseases In vivo hemic and lymphatic diseases Tumor Cells Cultured medicine Humans In vitro study Drug Interactions RNA Messenger Antineoplastic Agents Alkylating neoplasms Glutathione Transferase Ovarian Neoplasms Dose-Response Relationship Drug Biochemistry (medical) General Medicine Glutathione Blotting Northern In vitro Gene Expression Regulation Neoplastic chemistry Drug Resistance Neoplasm Female Cell Division Selenium medicine.drug |
| Zdroj: | Biological Trace Element Research. 65:187-195 |
| ISSN: | 1559-0720 0163-4984 |
| DOI: | 10.1007/bf02789095 |
| Popis: | Exposure of A2780 human ovarian tumor cells to a low concentration of melphalan in vitro for 7 d results in the development of melphalan resistance, which is dependent on elevated cellular levels of glutathione and glutathione S-transferase. The inclusion of selenite (at concentrations as low as 0.2 microM) during the exposure to melphalan completely prevented the development of resistance. Selenite did not prevent the melphalan-induced increase in glutathione, but it did prevent the increase in the activity of glutathione S-transferase. It also prevented the increase in the expression of the glutathione S-transferase gene, suggesting that this may be the mechanism by which it prevents the development of melphalan resistance. The results of this in vitro study suggest that selenite may prove to be useful in preventing the development of drug resistance in vivo. |
| Databáze: | OpenAIRE |
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