Analysis of vaginal microbicide film hydration kinetics by quantitative imaging refractometry

Autor: Adam Wax, Sheila Grab, Lisa C. Rohan, Matthew T. Rinehart, David F. Katz
Rok vydání: 2013
Předmět:
Viral Diseases
Polymers
Image Processing
Gynecologic Infections
Holography
lcsh:Medicine
law.invention
Immunodeficiency Viruses
law
lcsh:Science
chemistry.chemical_classification
Microscopy
Multidisciplinary
Obstetrics and Gynecology
Polymer
Infectious Diseases
Medical Microbiology
Viral Pathogens
Volume fraction
Vagina
Physical Sciences
Engineering and Technology
Female
Refractometry
Research Article
Quantitative imaging
Drug Research and Development
Materials by Structure
HIV prevention
Materials Science
Microbiology
Microbicide
Humans
Pharmacokinetics
Microbial Pathogens
Medicine and health sciences
Pharmacology
Preventive medicine
Mucous Membrane
Vaginal microbicide
lcsh:R
Biology and Life Sciences
HIV
Administration
Intravaginal
Public and occupational health
chemistry
Signal Processing
Anti-Infective Agents
Local
Women's Health
lcsh:Q
Clinical Medicine
Refractive index
Biomedical engineering
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 4, p e95005 (2014)
ISSN: 1932-6203
Popis: We have developed a quantitative imaging refractometry technique, based on holographic phase microscopy, as a tool for investigating microscopic structural changes in water-soluble polymeric materials. Here we apply the approach to analyze the structural degradation of vaginal topical microbicide films due to water uptake. We implemented transmission imaging of 1-mm diameter film samples loaded into a flow chamber with a 1.5×2 mm field of view. After water was flooded into the chamber, interference images were captured and analyzed to obtain high resolution maps of the local refractive index and subsequently the volume fraction and mass density of film material at each spatial location. Here, we compare the hydration dynamics of a panel of films with varying thicknesses and polymer compositions, demonstrating that quantitative imaging refractometry can be an effective tool for evaluating and characterizing the performance of candidate microbicide film designs for anti-HIV drug delivery.
Databáze: OpenAIRE
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