Label-free detection of aflatoxin M1 with electrochemical Fe3O4/polyaniline-based aptasensor
| Autor: | Phuc Xuan Nguyen, Quan Phuc Do, Lam Dai Tran, Binh Hai Nguyen, Ngoc Huan Tran, Huy Nguyen |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Aflatoxin Aniline Compounds Materials science Staining and Labeling Aptamer Reproducibility of Results Bioengineering Nanotechnology Biosensing Techniques Electrochemical Techniques Square wave Aptamers Nucleotide Electrochemistry Ferrosoferric Oxide Biomaterials chemistry.chemical_compound chemistry Mechanics of Materials Reagent Polyaniline Aflatoxin M1 Magnetic nanoparticles |
| Zdroj: | Materials Science and Engineering: C. 33:2229-2234 |
| ISSN: | 0928-4931 |
| Popis: | The selective detection of ultratrace amounts of aflatoxin M1 (AFM1) is extremely important for food safety since it is the most toxic mycotoxin class that is allowed to be present on cow milk with strictly low regulatory levels. In this work, Fe3O4 incorporated polyaniline (Fe3O4/PANi) film has been polymerized on interdigitated electrode (IDE) as sensitive film for AFM1 electrochemical biosensor. The immobilized aptamers as an affinity capture reagent and magnetic nanoparticles for signal amplification element have been employed in the sensing platform. Label-free and direct detection of the aptamer-AFM1 on Fe3O4/PANi interface were performed via electrochemical signal change, acquired by cyclic and square wave voltammetries. With a simplified strategy, this electrochemical aptasensor shows a good sensitivity to AFM1 in the range of 6-60 ng·L(-1), with the detection limit of 1.98 ng·L(-1). The results open up the path for designing cost effective aptasensors for other biomedical applications. |
| Databáze: | OpenAIRE |
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