A split aptamer-labeled ratiometric fluorescent biosensor for specific detection of adenosine in human urine
| Autor: | Shuhu Du, Tian Lu, Liying Zhang, Jiangrong Ji, Junhui You, Zhengyi You, Yuhong Xia, Lipin Wang, Xin Xu |
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| Rok vydání: | 2018 |
| Předmět: |
Fluorophore
Adenosine Aptamer Nanoprobe Color 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Fluorescence spectroscopy Fluorescence Analytical Chemistry chemistry.chemical_compound Quantum Dots medicine Fluorescence Resonance Energy Transfer Humans Detection limit Chromatography Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry 0210 nano-technology Biosensor medicine.drug |
| Zdroj: | Mikrochimica acta. 186(1) |
| ISSN: | 1436-5073 |
| Popis: | A dual-emission ratiometric fluorometric aptasensor is presented for highly specific detection of adenosine. An adenosine binding aptamer (ABA) was split into two halves (termed as ABA1 and ABA2). ABA1 was covalently bound to blue-emitting carbon dots (with excitation/emission maxima at 365/440 nm) as responsive fluorophore (referred to as ABA1-CDs). ABA2 was linked to red-emitting silica-coated CdTe quantum dots (with excitation/emission maxima at 365/613 nm) acting as internal reference and referred to as ABA2-QDs@SiO2. Upon addition of graphene oxide, the fluorescence of ABA1-CDs is quenched. After subsequent addition of ABA2-QDs@SiO2 and different amounts of adenosine, the blue fluorescence is recovered and causes a color change from red to royal blue. The method represents a ratiometric turn-on assay for visual, colorimetric and fluorometric determination of adenosine. The limit of detection is as low as 2.4 nM in case of ratiometric fluorometry. The method was successfully applied to the determination of adenosine in (spiked) human urine. Recoveries range from 98.8% to 102%. |
| Databáze: | OpenAIRE |
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