Long non-coding RNA OIP5-AS1 promotes the growth of gastric cancer through the miR-367-3p/HMGA2 axis
| Autor: | Yannan Wang, Caixia Sun, Xiaoyu Wan, Qihao Fan, Youmao Tao, Haojie Sun, Yuanyu Wu, Lushun Ma |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Carcinogenesis Apoptosis Mice 03 medical and health sciences 0302 clinical medicine HMGA2 Stomach Neoplasms Cell Line Tumor Animals Humans Medicine Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Mice Inbred BALB C Hepatology biology business.industry Cell growth HMGA2 Protein Gastroenterology Wnt signaling pathway Xenograft Model Antitumor Assays Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Cell culture 030220 oncology & carcinogenesis Disease Progression Cancer research biology.protein RNA Long Noncoding 030211 gastroenterology & hepatology business Signal Transduction |
| Zdroj: | Digestive and Liver Disease. 52:773-779 |
| ISSN: | 1590-8658 |
| DOI: | 10.1016/j.dld.2019.11.017 |
| Popis: | Increasing evidence shows that aberrant lncRNAs expression contributes to the progression of gastric cancer (GC). The role of the novel lncRNA OIP5-AS1 and its underlying mechanisms in the growth of GC is largely unknown. Here we demonstrate for the first time that OIP5-AS1 expression was up-regulated in GC tissues and cell lines, which significantly correlated with unfavorable clinical characteristics and shorter survival. The results of in vitro and in vivo gain- and loss-of-function experiments indicate that OIP5-AS1 promoted cell proliferation and colony formation while inhibiting apoptosis of GC cells. OIP5-AS1 functioned as an endogenous sponge for miR-367-3p in GC cells. Restoration of miR-367-3p expression abolished the biological effects of OIP5-AS1 on GC cells. Moreover, we show that HMGA2 was a downstream target of miR-367-3p and mediated the effects of OIP5-AS1 on GC cells. OIP5-AS1 regulated the activities of the PI3K/AKT and Wnt/β-catenin pathways through HMGA2. In conclusion, OIP5-AS1 functions as an oncogenic lncRNA that promotes the progression of GC and may serve as a therapeutic target for managing GC. |
| Databáze: | OpenAIRE |
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