Uremic serum-induced calcification of human aortic smooth muscle cells is a regulated process involving Klotho and RUNX2

Autor: Shori Thakur, Anwar R. Baydoun, Ken Farrington, Dhruv K. Singh, Ashish Patidar
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Serum
Parathyroid hormone
Core Binding Factor Alpha 1 Subunit
030204 cardiovascular system & hematology
urologic and male genital diseases
Biochemistry
Muscle
Smooth
Vascular
chemistry.chemical_compound
0302 clinical medicine
Osteogenesis
Chronic kidney disease
Receptor
Klotho
Aorta
Cells
Cultured
Research Articles
Vascular calcification
Glucuronidase
Aged
80 and over
Middle Aged
female genital diseases and pregnancy complications
Female
medicine.medical_specialty
RUNX2
Myocytes
Smooth Muscle
Biophysics
Renal function
03 medical and health sciences
Internal medicine
Uraemic serum
medicine
Animals
Humans
Human aortic smooth muscle Cells
Renal Insufficiency
Chronic
Vascular Calcification
Molecular Biology
Klotho Proteins
Aged
Uremia
business.industry
Cholesterol
Cell Biology
medicine.disease
Culture Media
Rats
030104 developmental biology
Endocrinology
chemistry
Cardiovascular System & Vascular Biology
Human aortic smooth muscle cells
business
Lipoprotein
Kidney disease
Calcification
Zdroj: Bioscience Reports
ISSN: 1573-4935
Popis: Vascular calcification (VC) is common in subjects with chronic kidney disease (CKD) and is associated with increased cardiovascular risk. It is an active process involving transdifferentiation of arterial smooth muscle cells (SMCs) into osteogenic phenotype. We investigated the ability of serum from CKD subjects to induce calcification in human SMCs in vitro (calcific potential of sera: CP), and associated changes in expression of Runt-related transcription factor 2 (RUNX2), SM22α, and Klotho. Sera from subjects with CKD (18 stage 3, 17 stage 4/5, and 29 stage 5D) and 20 controls were added to human cultured SMCs and CP quantified. The CP of CKD sera was greater (P
Databáze: OpenAIRE
Externí odkaz: