Screening of potential probiotic lactic acid bacteria and production of amylase and its partial purification
| Autor: | Rayavarapu Bhargavi, Purushothama Rao Priyanka, Naige Ranganath Niranjan, Tallapragada Padmavathi, Pogakul Veerabhadrappa Pavitra |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Lactobacillus fermentum lcsh:Biotechnology Antibiotic sensitivity Hydrophobicity 030106 microbiology Lb. fermentum law.invention 03 medical and health sciences Probiotic chemistry.chemical_compound law lcsh:TP248.13-248.65 General Materials Science Food science Amylase Polyacrylamide gel electrophoresis chemistry.chemical_classification Microbial/industrial Biotechnology biology Lactobacillus sp G3_4_1TO2 food and beverages biology.organism_classification Lactic acid lcsh:Genetics 030104 developmental biology Enzyme chemistry Low pH tolerance biology.protein Bile salt tolerance Bacteria |
| Zdroj: | Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 2, Pp 357-362 (2018) Journal of Genetic Engineering & Biotechnology |
| ISSN: | 1687-157X |
| DOI: | 10.1016/j.jgeb.2018.03.005 |
| Popis: | Probiotics are the healthy living bacteria when administered in adequate amounts confers health benefits in the host. The main objective of present study was to screen the bacteria for potential probiotic characters and enzyme production. The probiotic characters like tolerance to low pH, bile salts, antibiotic sensitivity, hydrophobicity and auto-aggregation properties were evaluated. Among all isolates Lactobacillus fermentum and Lactobacillus sp G3_4_1TO2 showed maximum potential probiotic characters and produced amylase enzyme by observing the halo zone around the colonies with the diameter 0.9 mm and 1.23 mm. Lactobacillus sp G3_4_1TO2 produced maximum amylase when compared with Lb. fermentum. The protein yield was 55.4% with the specific activity of 88.9 U/mg and obtained 40.8% purification fold. The molecular weight of amylase enzyme determined by SDS PAGE was 95,000 Da. From the present study it was considered that Lactobacillus sp G3_4_1TO2 was a potential probiotic bacteria producing maximum amylase enzyme. Keywords: Amylase, Bile salt tolerance, Hydrophobicity, Lb. fermentum, Low pH tolerance, Lactobacillus sp G3_4_1TO2 |
| Databáze: | OpenAIRE |
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