Localization and characterization of aminopeptidase P in bovine adrenal medulla
| Autor: | W.P. De Potter, J. De Block, G. Vanhoof, I. De Meester, Simon Scharpé |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
endocrine system
Cations Divalent Neuropeptide CD13 Antigens Biology Cell Fractionation Aminopeptidases Cellular and Molecular Neuroscience Cytosol Centrifugation Density Gradient medicine Animals Chromaffin Granules Protease Inhibitors Medulla Biological activity Cell Biology Hydrogen-Ion Concentration Kinetics Neuropeptide processing medicine.anatomical_structure Biochemistry Adrenal Medulla Chromaffin cell Cattle Adrenal medulla Subcellular Fractions Endocrine gland Cysteine |
| Zdroj: | Neurochemistry international |
| ISSN: | 0197-0186 |
| Popis: | Aminopeptidase P (EC 3.4.11.9) is demonstrated for the first time in the cytosolic fraction of chromaffin cells of the bovine adrenal medulla. The enzyme is inhibited by metal chelators and by sulfhydryl-reactive agents, which suggests that both a tightly bound metal ion and a cysteine residue are necessary for enzymatic activity. Aminopeptidase P might be important for the modulation of the biological activity of neuropeptides. Its occurrence in the adrenal chromaffin cells provides a useful tool for studying the function of this unique proline-specific peptidase in neuropeptide processing and secretion. |
| Databáze: | OpenAIRE |
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