Garcinia kola (Heckel) and Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg. from Cameroon possess potential antisalmonellal and antioxidant properties

Autor: Kouipou Rufin Marie Toghueo, Paul Keilah Lunga, Fred Djague, Dongmo Yanick Kevin Melogmo, Boyom Fabrice Fekam
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Bacterial Diseases
Phytochemistry
Leaves
Antioxidant
Salmonellosis
DPPH
medicine.medical_treatment
Phytochemicals
Cell Membranes
Plant Science
Pathology and Laboratory Medicine
Biochemistry
Salmonella Typhi
Antioxidants
Anthocyanins
chemistry.chemical_compound
Phytomedicine
Medical Conditions
Salmonella
Medicine and Health Sciences
Cameroon
Glycosides
Multidisciplinary
ABTS
Traditional medicine
biology
Plant Biochemistry
Nucleotides
Plant Anatomy
Euphorbiaceae
Antimicrobial
Bacterial Pathogens
Anti-Bacterial Agents
Chemistry
Infectious Diseases
Phytochemical
Medical Microbiology
Physical Sciences
Medicine
Pathogens
Cellular Structures and Organelles
Research Article
Alchornea cordifolia
Science
030106 microbiology
Garcinia kola
Microbial Sensitivity Tests
Microbiology
03 medical and health sciences
Enterobacteriaceae
medicine
Microbial Pathogens
Flavonoids
Bacteria
Plant Extracts
Organisms
Biology and Life Sciences
Cell Biology
biology.organism_classification
Triterpenes
030104 developmental biology
chemistry
Tannins
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 8, p e0237076 (2020)
ISSN: 1932-6203
Popis: Drug resistant Salmonella species and shortcomings related to current drugs stress the urgent need to search for new antimicrobial agents to control salmonellosis. This study investigated the antisalmonellal and antioxidant potentials of methanolic and hydro-ethanolic extracts of Garcinia kola and Alchornea cordifolia as potential sources of drugs to control Salmonella species and to reduce related oxidative stress. The antisalmonellal activity was assessed using the broth microdilution, membrane destabilization and time-kill kinetic assays. While, the DPPH, ABTS and FRAP assays were used for the determination of the antioxidant activities. The minimum inhibitory concentrations ranged from 125 to 1000 μg/mL, with the methanolic root extract of G. kola being the most active. The time kill kinetic assay revealed a concentration-dependent bacteriostatic activity for promising extracts. Potent extracts from G. kola showed the ability to destabilize S. typhi outer membrane, with the methanolic root extract presenting the highest activity; two-fold higher than those of polymyxin B tested as reference. In addition, this methanolic root extract of G. kola also provoked nucleotide leakage in a concentration-dependent manner. From the antioxidant assays, the hydro-ethanolic extract from the stem bark of A. cordifolia presented significant activities comparable to that of Vitamin C. The methanolic root extract of G. kola also presented appreciable antioxidant activities, though less than that of A. cordifolia. Overall, the phytochemical screening of active extracts revealed the presence of anthocyanins, flavonoids, glycosides, phenols, tannins, triterpenoids and steroids. These results provide evidence of the antibacterial potential of G. kola and offer great perspectives in a possible standardisation of an antisalmonellal phytomedicine.
Databáze: OpenAIRE
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