High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber
| Autor: | Kaori Niwa, Akira Mochizuki, Masashi Amano, Takanori Maeda, Yoshiyuki Imura, Keisuke Iwahori, Jiri Svoboda, Yumi Kawagoe |
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| Rok vydání: | 2013 |
| Předmět: |
Genetic Markers
Candidate gene DNA Plant Molecular Sequence Data Potyvirus Population Genes Recessive Locus (genetics) Genes Plant Real-Time Polymerase Chain Reaction Polymorphism Single Nucleotide Chromosomes Plant chemistry.chemical_compound Cucurbita Gene mapping Sequence Homology Nucleic Acid Molecular marker Genetics Amino Acid Sequence RNA Messenger education Gene Phylogeny Disease Resistance Plant Diseases education.field_of_study Zucchini yellow mosaic virus Base Sequence Sequence Homology Amino Acid biology Reverse Transcriptase Polymerase Chain Reaction Chromosome Mapping General Medicine biology.organism_classification Virology Phenotype chemistry Genetic marker Cucumis sativus Agronomy and Crop Science Microsatellite Repeats Biotechnology |
| Zdroj: | Theoretical and Applied Genetics. 126:2983-2993 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s00122-013-2187-5 |
| Popis: | Using a high-resolution mapping approach, we identified a candidate gene for ZYMV resistance in cucumber. Our findings should assist the development of high-versatility molecular markers for MAS for ZYMV resistance. Zucchini yellow mosaic virus (ZYMV) causes significant disease, which leads to fruit yield loss in cucurbit crops. Since ZYMV resistance is often inherited recessively in cucumber, marker-assisted selection (MAS) is a useful tool for the development of resistant cucumber cultivars. Using 128 families of an F2:3 population derived from a cross between susceptible ‘CS-PMR1’ and resistant ‘A192-18’ cucumber inbred lines, we confirmed that ZYMV resistance is conferred by a single recessive locus: zym A192-18 . We constructed a cucumber genetic linkage map that included 125 simple sequence repeat (SSR) markers segregating into 7 linkage groups (chromosomes). The zym A192-18 locus was mapped to chromosome 6, at genetic distances of 0.9 and 1.3 cM from two closely linked SSR markers. For high-resolution genetic mapping, we identified new molecular markers cosegregating with the zym A192-18 locus; using cucumber genomic and molecular marker resources and screening an F2 population of 2,429 plants, we narrowed down the zym A192-18 locus to a |
| Databáze: | OpenAIRE |
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