Consumption of Quercetin and Quercetin-Containing Apple and Cherry Extracts Affects Blood Glucose Concentration, Hepatic Metabolism, and Gene Expression Patterns in Obese C57BL/6J High Fat–Fed Mice
| Autor: | Bingxin Zhao, Debra K. Sullivan, George Cavender, Neil F. Shay, Ting Luo, C. Kaiser, Sarah M. Snyder, Jill Hamilton-Reeves |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Nutrition and Disease Peroxisome Proliferator-Activated Receptors Gene Expression Mice Obese Medicine (miscellaneous) Inflammation Prunus avium Biology Diet High-Fat 03 medical and health sciences chemistry.chemical_compound Internal medicine Diabetes mellitus medicine Animals Humans Obesity 030109 nutrition & dietetics Nutrition and Dietetics Plant Extracts food and beverages Polyphenols Hep G2 Cells Lipid Metabolism medicine.disease Dietary Fats Mice Inbred C57BL C-Reactive Protein 030104 developmental biology Endocrinology Liver Biochemistry Phytochemical chemistry Polyphenol Fruit Malus Quercetin Blood sugar regulation medicine.symptom Quantitative analysis (chemistry) Drug metabolism |
| Zdroj: | The Journal of Nutrition. 146:1001-1007 |
| ISSN: | 0022-3166 |
| DOI: | 10.3945/jn.115.228817 |
| Popis: | BACKGROUND Intake of polyphenols and polyphenol-rich fruit extracts has been shown to reduce markers of inflammation, diabetes, and hepatic complications that result from the consumption of a high-fat (HF) diet. OBJECTIVE The objective of this study was to determine whether mice fed polyphenol-rich apple peel extract (AE), cherry extract (CE), and quercetin, a phytochemical abundant in fruits including apples and cherries, would modulate the harmful effects of adiposity on blood glucose regulation, endocrine concentrations, and hepatic metabolism in HF-fed C57BL/6J male mice. METHODS Groups of 8-wk-old mice (n = 8 each) were fed 5 diets for 10 wk, including low-fat (LF; 10% of total energy) and HF (60% of total energy) control diets and 3 HF diets containing polyphenol-rich AE, CE, and quercetin (0.2% wt:wt). Also, an in vitro study used HepG2 cells exposed to quercetin (0-100 μmol/L) to determine whether intracellular lipid accumulation could be modulated by this phytochemical. RESULTS Mice fed the HF control diet consumed 36% more energy, gained 14 g more body weight, and had ∼50% elevated blood glucose concentrations (all P < 0.05) than did LF-fed mice. Mice fed HF diets containing AE, CE, or quercetin became as obese as HF-fed mice, but had significantly lower blood glucose concentrations after food deprivation (-36%, -22%, -22%, respectively; P < 0.05). Concentrations of serum C-reactive protein were reduced 29% in quercetin-fed mice compared with HF-fed controls (P < 0.05). A qualitative evaluation of liver tissue sections suggested that fruit phytochemicals may reduce hepatic lipid accumulation. A quantitative analysis of lipid accumulation in HepG2 cells demonstrated a dose-dependent decrease in lipid content in cells treated with 0-100 μmol quercetin/L (P < 0.05). CONCLUSIONS In mice, consumption of AE, CE, or quercetin appears to modulate some of the harmful effects associated with the consumption of an obesogenic HF diet. Furthermore, in a cell culture model, quercetin was shown to reduce intracellular lipid accumulation in a dose-dependent fashion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|