Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici
| Autor: | Bernardo Franco, Guadalupe Martínez-Cadena, Víctor Meza-Carmen, M. Isabel G. Roncero, Carlos A. Araiza-Cervantes, Georgina E. Reyna-López |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Repair Glycoside Hydrolases DNA repair Genes Fungal Virulence medicine.disease_cause Microbiology 03 medical and health sciences Fusarium Fusarium oxysporum medicine Amino Acid Sequence Molecular Biology Escherichia coli Gene PARG Mutation biology food and beverages General Medicine Sequence Analysis DNA biology.organism_classification 030104 developmental biology Fusarium oxysporum f.sp. lycopersici Genome Fungal DNA Damage |
| Zdroj: | Antonie van Leeuwenhoek. 111(2) |
| ISSN: | 1572-9699 |
| Popis: | The genome sequence of the plant pathogen Fusarium oxysporum f. sp. lycopersici contains a single gene encoding a predicted poly(ADP-ribose) glycohydrolase (FOXG_05947.2, PARG). Here, we assessed whether this gene has a role as a global regulator of DNA repair or in virulence as an ADP ribosylating toxin homologue of bacteria. The PARG protein was purified after expressing its encoding gene in Escherichia coli. Its inhibition by 6,9-diamino-2-ethoxyacridine lactate monohydrate and tannins was similar to its human orthologue that is involved in DNA repair. A deletion strain of F. oxysporum f. sp. lycopersici showed no growth defects and was not affected in pathogenicity. Together, our results indicate that the PARG protein of F. oxysporum f. sp. lycopersici is involved in DNA repair and does not act in pathogenicity as an effector. |
| Databáze: | OpenAIRE |
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