Genetic Diversity of the Hepatitis B Virus Strains in Cuba: Absence of West-African Genotypes despite the Transatlantic Slave Trade
| Autor: | María Caridad Montalvo Villalba, Claude P. Muller, Meilin S. Wong, Susel Sariego Frómeta, Aurélie Sausy, Marité Bello Corredor, Judith M. Hübschen, Licel de los Ángeles Rodríguez Lay, Marcia Samada, Lidunka Valdes |
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| Rok vydání: | 2015 |
| Předmět: |
Serotype
Hepatitis B virus HBsAg Genotype Science Molecular Sequence Data Population Gene mutation Biology medicine.disease_cause Genetic variation medicine Humans education Phylogeny Genetics education.field_of_study Genetic diversity Multidisciplinary Cuba Genetic Variation Virology Medicine Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 5, p e0125052 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Cuba is an HBsAg low-prevalence country with a high coverage of anti-hepatitis B vaccine. Its population is essentially the result of the population mix of Spanish descendants and former African slaves. Information about genetic characteristics of hepatitis B virus (HBV) strains circulating in the country is scarce. The HBV genotypes/subgenotypes, serotypes, mixed infections, and S gene mutations of 172 Cuban HBsAg and HBV-DNA positive patients were determined by direct sequencing and phylogenetic analysis. Phylogenetic analysis of HBV S gene sequences showed a predominance of genotype A (92.4%), subgenotype A2 (84.9%) and A1 (7.6%). Genotype D (7.0%) and subgenotype C1 (0.6%) were also detected but typical (sub)genotypes of contemporary West-Africa (E, A3) were conspicuously absent. All genotype A, D, and C strains exhibited sequence characteristics of the adw2, ayw2, and adrq serotypes, respectively. Thirty-three (19.1%) patients showed single, double, or multiple point mutations inside the Major Hydrophilic domain associated with vaccine escape; eighteen (10.5%) patients had mutations in the T-cell epitope (amino acids 28-51), and there were another 111 point mutations downstream of the S gene. One patient had an HBV A1/A2 mixed infection. This first genetic study of Cuban HBV viruses revealed only strains that were interspersed with strains from particularly Europe, America, and Asia. The absence of genotype E supports previous hypotheses about an only recent introduction of this genotype into the general population in Africa. The presence of well-known vaccine escape (3.5%) and viral resistance mutants (2.9%) warrants strain surveillance to guide vaccination and treatment strategies. |
| Databáze: | OpenAIRE |
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