Long-Term Effects With Potential Clinical Importance of Botulinum Toxin Type-A on Mechanics of Muscles Exposed
| Autor: | Zeynep D. Akdeniz-Doğan, Can A. Yucesoy, Evrim O. Yılmaz, Cemre S. Kaya |
|---|---|
| Přispěvatelé: | Kaya, Cemre S., Yilmaz, Evrim O., Akdeniz-Dogan, Zeynep D., Yucesoy, Can A. |
| Rok vydání: | 2020 |
| Předmět: |
collagen
0301 basic medicine Histology SYNERGISTIC MUSCLES NEUROTOXIN TYPE-A lcsh:Biotechnology medicine.medical_treatment MYOFASCIAL FORCE TRANSMISSION CEREBRAL-PALSY Biomedical Engineering LIMB SPASTICITY Bioengineering 02 engineering and technology Isometric exercise DOUBLE-BLIND 03 medical and health sciences Hydroxyproline chemistry.chemical_compound MUSCULAR MECHANICS lcsh:TP248.13-248.65 MAGNETIC-RESONANCE medicine Exertion Spasticity Saline Original Research active force Passive resistance animal model Compartment (ship) Bioengineering and Biotechnology Mechanics 021001 nanoscience & nanotechnology Botulinum toxin botulinum toxin type A muscle mechanical function passive force 030104 developmental biology chemistry ACTIVATED SPASTIC SEMITENDINOSUS medicine.symptom MANUAL NEEDLE PLACEMENT 0210 nano-technology Biotechnology medicine.drug |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| DOI: | 10.3389/fbioe.2020.00738 |
| Popis: | Botulinum toxin type-A (BTX-A) is widely used for spasticity management and mechanically aims at reducing passive resistance at the joint and widening joint range of movement. However, recent experiments on acute BTX-A effects showed that the injected rat tibialis anterior (TA) muscle's passive forces increased, and the length range of active force exertion (l(range)) did not change. Additionally, BTX-A was shown to spread into non-injected muscles in the compartment and affect their mechanics. Whether those effects persist in the long term is highly important, but unknown. The aim was to test the following hypotheses with experiments conducted in the anterior crural compartment of the rat: In the long term, BTX-A (1) maintains l(range), (2) increases passive forces of the injected TA muscle, and (3) spreads into non-injected extensor digitorum longus (EDL) and the extensor hallucis longus (EHL) muscles, also affecting their active and passive forces. Male Wistar rats were divided into two groups: BTX-A and Control (0.1 units of BTX-A or only saline was injected into the TA). Isometric forces of the muscles were measured simultaneously 1-month post-injection. The targeted TA was lengthened, whereas the non-targeted EDL and EHL were kept at constant length. Hydroxyproline analysis was done to quantify changes in the collagen content of studied muscles. Two-way ANOVA test (for muscle forces, factors: TA length and animal group) and unpaired t or Mann-Whitney U test (for l(range) and collagen content, where appropriate) were used for statistical analyses (P < 0.05). BTX-A caused significant effects. TA: active forces decreased (maximally by 75.2% at short and minimally by 48.3%, at long muscle lengths), l(range) decreased (by 22.9%), passive forces increased (by 12.3%), and collagen content increased (approximately threefold). EDL and EHL: active forces decreased (up to 66.8%), passive force increased (minimally by 62.5%), and collagen content increased (approximately twofold). Therefore, hypothesis 1 was rejected and 2 and 3 were confirmed indicating that previously reported acute BTX-A effects persist and advance in the long term. A narrower l(range) and an elevated passive resistance of the targeted muscle are unintended mechanical effects, whereas spread of BTX-A into other compartmental muscles indicates the presence of uncontrolled mechanical effects. |
| Databáze: | OpenAIRE |
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