Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material
| Autor: | Shin'ichiro Kajiyama, Shigeki Kawakami, Kiichi Fukui, Tomoko Murakawa, Tomohiko Ikeuchi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Liposome
Phosphatidylethanolamines Protoplasts Genetic transfer Green Fluorescent Proteins Gene Transfer Techniques Bioengineering Gene transfer DNA Protoplast Biology Plants Applied Microbiology and Biotechnology chemistry.chemical_compound Transformation (genetics) Transformation Genetic Biochemistry chemistry Naked DNA Caulimovirus Biophysics Promoter Regions Genetic Biotechnology Transformation efficiency |
| Zdroj: | Journal of bioscience and bioengineering. 105(1) |
| ISSN: | 1389-1723 |
| Popis: | The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect