Technique for the preparation of homogeneous cultures of isolated heart muscle cells
| Autor: | O.M. Bucher, E. Schöpf-Ebner, W.O. Gross |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Agglutination
Erythrocytes Myocardium Embryo Chick Embryo Cell Biology Isolated heart Biology Antibodies Culture Media Rats Mice Agglutination (biology) Blood Biochemistry Homogeneous Culture Techniques Lactalbumin hydrolysate Methods Homologous chromosome Animals Yeast extract Myocyte Cell Aggregation |
| Zdroj: | Experimental Cell Research. 53:1-10 |
| ISSN: | 0014-4827 |
| Popis: | Experiments with trypsin-dissociated heart muscle cells have shown two distinct processes, aggregation and agglutination , to be involved in the socalled reaggregation of cells. In the case of aggregation the cells adhere to one another by products which originate from them. The cells lose their capacity to conglomerate once they have been washed several times with the nutrient solution. The agglutination is caused by constituents of the nutrient medium. In the calf sera used by us as admixtures to the lactalbumin hydrolysate + yeast extract + Hanks solution, three different agglutinins against heart muscle cells of chick embryo, rat, and mouse were identified. The agglutination of isolated myocardial cells by the nutrient medium can be avoided by previously absorbing the agglutinins out of the serum onto homologous erythrocytes. The prevention of aggregation and agglutination on preparing the culture as well as the further feeding with a medium deprived of agglutinins were prerequisites for the culture condition under which many of the heart muscle cells pulsated for two months and several, in two preparations, still on the 114th and 188th day. |
| Databáze: | OpenAIRE |
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