Ski-interacting protein, a bifunctional nuclear receptor coregulator that interacts with N-CoR/SMRT and p300
| Autor: | John A. Eisman, Edith M. Gardiner, Michael J. Hayman, Nanthakumar Subramaniam, Gary M. Leong, Janelle B. Barry, Tomoshige Kino, Laura L. Issa, Paul H. Driggers |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Transcriptional Activation
congenital hereditary and neonatal diseases and abnormalities Transcription Genetic Receptors Retinoic Acid Recombinant Fusion Proteins Biophysics Gene Expression Receptors Cytoplasmic and Nuclear Repressor Cell Cycle Proteins P300-CBP Transcription Factors Retinoid X receptor Biology Biochemistry Calcitriol receptor Cell Line Mice Acetyltransferases Chlorocebus aethiops Coactivator Animals Nuclear Receptor Co-Repressor 2 p300-CBP Transcription Factors Molecular Biology Histone Acetyltransferases Sequence Deletion Nuclear receptor co-repressor 2 Nuclear Proteins Cell Biology Molecular biology DNA-Binding Proteins Repressor Proteins Retinoid X Receptors Nuclear receptor COS Cells NIH 3T3 Cells Receptors Calcitriol Corepressor Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 315:1070-1076 |
| ISSN: | 0006-291X |
| Popis: | Ski-interacting protein (SKIP), a vitamin D receptor (VDR) coactivator, also functions as a repressor in Notch signalling in association with the corepressor SMRT. Here we show that SKIP bifunctionally modulates (activates or represses) Retinoid-X receptor (RXR)- and VDR-dependent gene transcription in a cell line-specific manner, with activation in CV-1 and repression in P19 cells. The coactivator function of SKIP in these cells appeared to correlate with the relative level and ratio of expression of N-CoR and p300, with greater SKIP activation in higher p300-expressing and lower N-CoR-expressing cell-lines. C-terminal deletion of SKIP (delta334-536 aa) was associated with strong activation in both CV-1 and P19 cells. The corepressors N-CoR and SMRT and the coregulator p300 interacted with SKIP through the same N-terminal region (1-200 aa). Overall these results suggest that transcriptional action of SKIP may depend on distinct functional domains and cell line-specific interactions with both corepressors and coactivators. |
| Databáze: | OpenAIRE |
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