Additional file 2 of PCGF6/MAX/KDM5D facilitates MAZ/CDK4 axis expression and pRCC progression by hypomethylation of the DNA promoter

Autor: Zhu, Meng, Zhang, Ruo-Nan, Zhang, Hong, Qu, Chang-bao, Zhang, Xiao-chong, Ren, Li-Xin, Yang, Zhan, Gu, Jun-Fei
Rok vydání: 2023
DOI: 10.6084/m9.figshare.22625320
Popis: Additional file 2. Fig. S1: A Correlation analysis was performed between MAZ and PCGF6 mRNA expression in pRCC tissues from the data of TCGA. Fig. S2: RT-qPCR and Western blot were used to verify MAZ expression. A Caki-2 cells were transfected with MAZ shRNAs and ACHN cells were transfected with MAZ overexpression vectors. RT-qPCR was conducted to measure the mRNA level of MAZ. B The indicated vectors were transfected into cells as A, and the expression of MAZ was measured using Western blotting. C Quantitative analysis of Western blotting from B. Fig. S3: Correlation analysis was performed between MAZ promoter hypo-methylation and PCGF6 mRNA expression in pRCC tissues. Fig. S4: ACHN cells co-transfected with the indicated vectors were used for the luciferase reporter assays. **p < 0.01 versus the corresponding controls. Fig. S5: Luciferase reporter assays were performed to detect CDK4 promoter activity in Caki-2 cells after cotransfected with indicated vectors.
Databáze: OpenAIRE