Inhibitors of protein kinase C. 2. Substituted bisindolylmaleimides with improved potency and selectivity
| Autor: | Elizabeth Keech, Harris William, M. K. Hari Kumar, John S. Nixon, L H Elliott, Steven A. Hurst, Sandra E. Wilkinson, Geoffrey Lawton, Hill Christopher Huw, Davis Peter David |
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| Rok vydání: | 1992 |
| Předmět: |
Models
Molecular Stereochemistry Maleimides Structure-Activity Relationship Drug Discovery medicine Structure–activity relationship Staurosporine Potency Animals Humans Protein kinase A Protein kinase C Protein Kinase C chemistry.chemical_classification biology Chemistry Biological activity Rats Enzyme Biochemistry Enzyme inhibitor biology.protein Molecular Medicine Cattle Female medicine.drug |
| Zdroj: | Journal of medicinal chemistry. 35(6) |
| ISSN: | 0022-2623 |
| Popis: | A hypothetical mode of inhibition of protein kinase C (PKC) by the natural product staurosporine has been used as a basis for the design of substituted bisindolylmaleimides with improved potency over the parent compound. Structure-activity relationships were consistent with the interaction of a cationic group in the inhibitor with a carboxylate group in the enzyme, and the most potent compound had a Ki of 3 nM. The inhibitors were competitive with ATP but inhibited cAMP-dependent protein kinase (PKA) only at much higher concentrations despite the extensive sequence homology between the ATP-binding regions of PKA and PKC. Three compounds were evaluated further and found to inhibit a human allogeneic mixed lymphocyte reaction pointing to the potential utility of PKC inhibitors in immunosuppressive therapy. One of these compounds was orally absorbed in the rat and represents an attractive lead in the development of PKC inhibitors as drugs. |
| Databáze: | OpenAIRE |
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