Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro-matured domestic cat (Felis catus) oocytes when using ICSI
| Autor: | Jennifer Ringleb, K. Jewgenow, R. Waurich, Gudrun Wibbelt, W. J. Streich |
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| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system Sperm Retrieval medicine.medical_treatment Reproductive technology Fertilization in Vitro Biology Breeding Cryopreservation Intracytoplasmic sperm injection Specimen Handling Andrology Endocrinology Pregnancy Genetics medicine Animals Sperm Injections Intracytoplasmic Molecular Biology reproductive and urinary physiology Fertilisation urogenital system Embryo culture Anatomy Sperm bank Sperm Spermatozoa Reproductive Medicine Fertilization Cats Microscopy Electron Scanning Oocytes Animal Science and Zoology Female Spermatogenesis Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 23(6) |
| ISSN: | 1031-3613 |
| Popis: | The impact of different storage conditions of epididymal spermatozoa (including prolonged storage, cryopreservation and freeze-drying) on their fertilisation capacity was tested using intracytoplasmic sperm injection (ICSI). This kind of information is urgently needed when applying assisted reproductive technology to endangered felids in zoos. In particular, the utilisation of epididymal spermatozoa of castrated or deceased felids often requires time-consuming transportation and is therefore susceptible to loss of gamete quality. Sperm cells were stored at 4°C for up to 72 h followed by cryopreservation or freeze-drying. Thawed motile and immotile spermatozoa were used for ICSI and the embryo cleavage rate was assessed 36 h after injection. A significant impact on the fertilisation rate of oocytes could only be detected when using immotile thawed or rehydrated spermatozoa. Cryopreservation or storage at 4°C showed no influence. The simulation of transport conditions using domestic cat spermatozoa revealed that in vitro production of felid embryos with gametes from euthanised individuals is possible if testes are stored cool and arrive at the laboratory within 72 h. An essential prerequisite is the application of ICSI to achieve fertilisation even with single motile spermatozoa. Additional cryopreservation of spermatozoa after transportation is possible and will allow the establishment of a sperm bank for felids. |
| Databáze: | OpenAIRE |
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