Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts▿
| Autor: | Laura Ramírez, Vinicio T.S. Coelho, C.G. Nieto, Manuel Soto, Eduardo A.F. Coelho, Daniel R. Abánades, Pedro Bonay, Mariana Amalia Figueiredo Costa, Miguel A. Chávez-Fumagalli, Vívian T. Martins, Dulcilene Mayrink de Oliveira, Carlos Alonso, Carlos Alberto Pereira Tavares |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Fundación Ramón Areces |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Ribosomal Proteins Antigenicity Clinical Biochemistry Immunology Ribosomal proteins Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Cross Reactions Veterinary Immunology Serology Dogs Antigen Diagnosis parasitic diseases medicine Immunology and Allergy Animals Serologic Tests Dog Diseases Leishmania infantum Leishmania biology Leishmania chagasi biology.organism_classification medicine.disease Virology Visceral leishmaniasis biology.protein Leishmaniasis Visceral lipids (amino acids peptides and proteins) Antibody |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | In the present work, we have analyzed the antigenicity of Leishmania species ribosomal proteins (LRPs). To accomplish this, Leishmania infantum ribosomes were biochemically purified from promastigote cytosolic extracts, and their reactivities were analyzed by using the sera from dogs naturally infected with L. infantum. Since antibodies reacting against different ribosomal proteins were observed in all the serum samples obtained from dogs with symptomatic visceral leishmaniasis tested, we have analyzed the potential usefulness of the LRP extracts in the development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of canine visceral leishmaniasis (CVL) in an area of Brazil where visceral leishmaniasis is endemic due to infection by Leishmania chagasi. A comparative ELISA with crude soluble Leishmania chagasi antigen (SLA) and L. infantum LRPs was performed. LRP- and SLA-based ELISAs gave similar sensitivities for the diagnosis of symptomatic CVL, but the LRP extract provided a very high sensitivity for the detection of oligosymptomatic and asymptomatic dogs. In addition, an LRP-based ELISA showed a higher specificity when the sera from dogs harboring other infections were included in the analysis. The LRP antigen displayed no cross-reactivity with sera from dogs that had any of the other diseases tested, notably, Chagas’ disease. Our findings suggest that LRPs are a potential tool for the diagnosis of CVL and will be particularly useful for the diagnosis of asymptomatic CVL. This study was supported by grant FIS/PI080101 from the Ministerio de Ciencia e Innovacio´n and grant RICET RD06/0021/0008 from the Instituto de Salud Carlos III within the Network of Tropical Diseases Research. An institutional grant from Fundacio´n Ramo´n Areces is also acknowledged. |
| Databáze: | OpenAIRE |
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