CE-MSn of complex pectin-derived oligomers
| Autor: | Alphons G. J. Voragen, G.J. Coenen, Henk A. Schols, Mirjam A. Kabel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
anion-exchange chromatography
hairy ramified regions food.ingredient Pectin Clinical Biochemistry Mass spectrometry Biochemistry Oligomer Mass Spectrometry Analytical Chemistry Hydrolysis chemistry.chemical_compound food Capillary electrophoresis oligosaccharides Levensmiddelenchemie Organic chemistry galacturonic acid VLAG Chromatography Pyrenes Ion exchange Molecular Structure Food Chemistry Chemistry Elution Electrophoresis Capillary 8-aminonaphthalene-1 Hydrogen-Ion Concentration trap mass-spectrometry Matrix-assisted laser desorption/ionization apple pectin maldi-tof ms 6-trisulfonic acid 8-aminonaphthalene-1 3 6-trisulfonic acid Malus capillary zone electrophoresis Pectins rhamnogalacturonan-i |
| Zdroj: | Electrophoresis, 29(10), 2101-2111 Electrophoresis 29 (2008) 10 |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.200700465 |
| Popis: | As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high-performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE-IT-MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low-pH CE-LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE-MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE-MSn. Oligomers present in low amounts could be localized by their corresponding m/z, as was demonstrated by selected mass range representation. |
| Databáze: | OpenAIRE |
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