Diffusion-Limited Growth of Microbial Colonies
| Autor: | J. E. F. Green, Stephen G. Oliver, Jennifer M. Gardner, Zoltán Szenczi, Joanna F. Sundstrom, Benjamin J. Binder, Hayden Tronnolone, Sanjeeva Balasuriya, Ee Lin Tek, Vladimir Jiranek, Alexander Tam |
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| Přispěvatelé: | Tronnolone, Hayden, Tam, Alexander, Szenczi, Zoltán, Green, JEF, Balasuriya, Sanjeeva, Tek, Ee Lin, Gardner, Jennifer M, Sundstrom, Joanna F, Jiranek, Vladimir, Oliver, Stephen G, Binder, Benjamin J, Tronnolone, Hayden [0000-0003-4532-2030], Tam, Alexander [0000-0003-3565-1068], Green, JEF [0000-0001-5061-9563], Balasuriya, Sanjeeva [0000-0002-3261-7940], Tek, Ee Lin [0000-0001-7441-6840], Gardner, Jennifer M [0000-0002-4215-955X], Sundstrom, Joanna F [0000-0002-4898-3101], Jiranek, Vladimir [0000-0002-9775-8963], Oliver, Stephen G [0000-0003-3410-6439], Binder, Benjamin J [0000-0002-1812-6715], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae lcsh:Medicine Bacillus subtilis Bacterial growth 01 natural sciences microbial colonies Models Biological Article Diffusion 03 medical and health sciences Solid substrate Pseudohyphal growth 0103 physical sciences Computer Simulation Diffusion (business) 010306 general physics lcsh:Science Multidisciplinary biology Chemistry fungi lcsh:R biology.organism_classification Yeast diffusion-limited growth Multidisciplinary Sciences 030104 developmental biology DLG Biophysics lcsh:Q Bacteria Algorithms |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The emergence of diffusion-limited growth (DLG) within a microbial colony on a solid substrate is studied using a combination of mathematical modelling and experiments. Using an agent-based model of the interaction between microbial cells and a diffusing nutrient, it is shown that growth directed towards a nutrient source may be used as an indicator that DLG is influencing the colony morphology. A continuous reaction–diffusion model for microbial growth is employed to identify the parameter regime in which DLG is expected to arise. Comparisons between the model and experimental data are used to argue that the bacterium Bacillus subtilis can undergo DLG, while the yeast Saccharomyces cerevisiae cannot, and thus the non-uniform growth exhibited by this yeast must be caused by the pseudohyphal growth mode rather than limited nutrient availability. Experiments testing directly for DLG features in yeast colonies are used to confirm this hypothesis. |
| Databáze: | OpenAIRE |
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