Transcription of hexose transporters of Saccharomyces cerevisiae is affected by change in oxygen provision
| Autor: | Eija Rintala, Marilyn G. Wiebe, Merja Penttilä, Laura Ruohonen, Anu Tamminen |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Snf3 Saccharomyces cerevisiae Proteins Monosaccharide Transport Proteins Transcription Genetic Glucose uptake Saccharomyces cerevisiae lcsh:QR1-502 chemistry.chemical_element Chemostat Biology Microbiology Oxygen lcsh:Microbiology Hexose Anaerobiosis Promoter Regions Genetic chemistry.chemical_classification Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Aerobiosis Glucose chemistry Biochemistry Multigene Family Limiting oxygen concentration Anaerobic exercise Research Article |
| Zdroj: | Rintala, E, Wiebe, M G, Tamminen, A, Ruohonen, L & Penttilä, M 2008, ' Transcription of hexose transporters of Saccharomyces cerevisiae is affected by change in oxygen provision ', BMC Microbiology, vol. 8, 53 . https://doi.org/10.1186/1471-2180-8-53 BMC Microbiology BMC Microbiology, Vol 8, Iss 1, p 53 (2008) |
| Popis: | Background The gene family of hexose transporters in Saccharomyces cerevisiae consists of 20 members; 18 genes encoding transporters (HXT1-HXT17, GAL2) and two genes encoding sensors (SNF3, RGT2). The effect of oxygen provision on the expression of these genes was studied in glucose-limited chemostat cultivations (D = 0.10 h-1, pH 5, 30°C). Transcript levels were measured from cells grown in five steady state oxygen levels (0, 0.5, 1, 2.8 and 20.9% O2), and from cells under conditions in which oxygen was introduced to anaerobic cultures or removed from cultures receiving oxygen. Results The expression pattern of the HXT gene family was distinct in cells grown under aerobic, hypoxic and anaerobic conditions. The transcription of HXT2, HXT4 and HXT5 was low when the oxygen concentration in the cultures was low, both under steady state and non-steady state conditions, whereas the expression of HXT6, HXT13 and HXT15/16 was higher in hypoxic than in fully aerobic or anaerobic conditions. None of the HXT genes showed higher transcript levels in strictly anaerobic conditions. Expression of HXT9, HXT14 and GAL2 was not detected under the culture conditions studied. Conclusion When oxygen becomes limiting in a glucose-limited chemostat cultivation, the glucose uptake rate per cell increases. However, the expression of none of the hexose transporter encoding genes was increased in anaerobic conditions. It thus seems that the decrease in the moderately low affinity uptake and consequently the relative increase of high affinity uptake may itself allow the higher specific glucose consumption rate to occur in anaerobic compared to aerobic conditions. |
| Databáze: | OpenAIRE |
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