Oxidative DNA damage measured in human lymphocytes: large differences between sexes and between countries, and correlations with heart disease mortality rates
| Autor: | Susan Southon, Catherine M. Gedik, B. Olmedilla, M. C. Bellizzi, Andrew Collins |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
chemistry.chemical_classification
Antioxidant Vitamin C Heart disease Vitamin E medicine.medical_treatment Lymphocyte Cancer Physiology Biology medicine.disease Biochemistry High-performance liquid chromatography medicine.anatomical_structure chemistry Immunology Genetics medicine Molecular Biology Carotenoid Biotechnology |
| Zdroj: | ResearcherID |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fasebj.12.13.1397 |
| Popis: | The ‘antioxidant hypothesis’ proposes that vitamin C, vitamin E, carotenoids, and other antioxidants occurring in fruit and vegetables afford protection against heart disease and cancer by preventing oxidative damage to lipids and to DNA, respectively. To test elements of this hypothesis, we have measured blood levels of dietary antioxidants, and 8-oxodeoxyguanosine (8-oxo-dG) concentrations in lymphocyte DNA, in healthy men and women from five European countries: France, Ireland, The Netherlands, Spain, and the U.K. Volunteers, aged 25–45, all nonsmokers, gave blood samples before and after a 12-wk carotenoid supplementation regime. Vitamin C was measured in plasma and vitamin E and carotenoids were measured in serum by high-performance liquid chromatography (HPLC). 8-oxo-dG was assayed by HPLC (with coulometric detection) in DNA isolated from lymphocytes from the same blood samples. Mean values were calculated for groups of volunteers at each sampling time according to country, sex, and supplementation ... |
| Databáze: | OpenAIRE |
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