Docosahexaenoic Acid Modulates Invasion and Metastasis of Human Ovarian Cancer via Multiple Molecular Pathways
| Autor: | Yi-Nan Wu, Su-Li Wang, Ying-Chun Wang, Jin-Hua Wang, Ming-Fang He, Qiao-lin Liu, Qing-Hua Lin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Docosahexaenoic Acids Cell Survival Cell Down-Regulation Biology Metastasis Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Gentamicin protection assay Western blot Basic Science Invasion Ovarian cancer Internal medicine Cell Line Tumor medicine Animals Humans Neoplasm Invasiveness RNA Messenger Neoplasm Metastasis Cytotoxicity Zebrafish Ovarian Neoplasms medicine.diagnostic_test Obstetrics and Gynecology Cancer medicine.disease Xenograft Model Antitumor Assays Docosahexaenoic acid 030104 developmental biology medicine.anatomical_structure Endocrinology Oncology 030220 oncology & carcinogenesis Cancer research Female |
| Zdroj: | International Journal of Gynecological Cancer |
| ISSN: | 1525-1438 |
| Popis: | ObjectiveWe investigated the effect of docosahexaenoic acid (DHA) on the invasion and metastasis of ovarian cancer cells (A2780, HO8910, and SKOV-3).MethodsCytotoxicity assay was performed to determine the optimal doses of DHA in this experiment. The effects of DHA on invasion ability were assessed by invasion assay. The expressions of messenger RNA and/or proteins associated with invasion or metastasis were detected by quantitative Real Time-Polymerase Chain Reaction or Western blot. The effect of DHA on cell metastasis was assessed in xenograft model of zebrafish.ResultsDocosahexaenoic acid and α-linolenic acid could reduce the cell vitalities in dose-dependent manner. However, DHA inhibited the invasion and metastasis of ovarian cancer cells, but α-linolenic acid did not (**P < 0.01). Docosahexaenoic acid could downregulate the expressions of WAVE3, vascular endothelial cell growth factor, and MMP-9, and upregulate KISS-1, TIMP-1, and PPAR-γ, which negatively correlated with cell invasion and metastasis (*P < 0.05). Docosahexaenoic acid restrained the development of subintestinal vessels and cancer cell metastasis in xenograft model of zebrafish (**P < 0.01).ConclusionsDocosahexaenoic acid inhibited the invasion and metastasis of ovarian cancer cells in vitro and in vivo through the modulation of NF-κB signaling pathway, suggesting that DHA is a promising candidate for ovarian cancer therapy. |
| Databáze: | OpenAIRE |
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