Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5)
| Autor: | C. Stähli, H. Carmann, J. von Overbeck, Ursula Dürmüller, Ph. U. Heitz, V. Miggiano, Fred Gudat, C. Lautenschlager, Th. Staehelin, Bela Takacs |
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| Rok vydání: | 1985 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.drug_class Fluorescent Antibody Technique Monoclonal antibody Epithelium Pathology and Forensic Medicine Immunoenzyme Techniques Epitopes Mice Antigen Neoplasms Freezing medicine Animals Humans Molecular Biology Mice Inbred BALB C Frozen section procedure biology Chemistry Carcinoma Histological Techniques Mesenchymal stem cell Antibodies Monoclonal Epithelial Cells Cell Biology General Medicine Molecular biology Paraffin Immunoglobulin G Monoclonal biology.protein Immunohistochemistry Female Antibody Clone (B-cell biology) |
| Zdroj: | Virchows Archiv A Pathological Anatomy and Histopathology. 407:1-12 |
| ISSN: | 1432-2307 0174-7398 |
| DOI: | 10.1007/bf00701324 |
| Popis: | A mouse monoclonal antibody (mAB lu-5) was prepared using a lung cancer cell line as an antigen. The selected clone produces an IgG with a gamma-1 heavy chain and a kappa-light-chain. Immunohistochemical testing of mAB lu-5 on 117 normal tissue biopsies and 474 tumours revealed reactivity with an intracytoplasmic, formaldehyderesistant antigen present in most epithelial and mesothelial cells, but absent in mesenchymal cells. The antibody can therefore be used as a first order, pan-epithelial marker. It proved also useful for fast tumour diagnosis on frozen sections. |
| Databáze: | OpenAIRE |
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