Characterization of a novel strain of Aspergillus aculeatinus: From rhamnogalacturonan type I pectin degradation to improvement of fruit juice filtration
| Autor: | Magali Wagner, Aurore Perrin, Yves Coutel, Pascal Le Blond, Anaïs Guillaume, Yoann Carré, Corinne Pau-Roblot, Catherine Fourtot-Brun, Olivier Habrylo, Catalina Duran Garzon, Loïc Levavasseur, Jérôme Pelloux, Adrien Lemaire, Pauline Trezel, Stéphane Georgé, Clémence Millet |
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| Přispěvatelé: | Groupe Soufflet, Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI), Université de Picardie Jules Verne (UPJV)-Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Université d'Artois (UA)-Université de Liège-Université de Picardie Jules Verne (UPJV)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Université d'Artois (UA)-Université de Liège-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Transfrontalière BioEcoAgro - UMR 1158 (BioEcoAgro), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Centre Technique de la Conservation des Produits Agricoles |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Proteome Glycoside Hydrolases Polymers and Plastics Food Handling Hydrolases [SDV]Life Sciences [q-bio] RGI-type pectins-degrading enzymes 02 engineering and technology Cellulase 010402 general chemistry 01 natural sciences Fungal Proteins Cell wall Hydrolysis Solid-state fermentation Rhamnogalacturonan acetylesterase Rhamnogalacturonans Materials Chemistry Cellulases Aspergillus aculeatinus Food science chemistry.chemical_classification Aspergillus biology Juice filtration Chemistry Organic Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Fruit and Vegetable Juices Polygalacturonase Enzyme Mucilage Malus biology.protein Carbohydrate Metabolism Pectins 0210 nano-technology Filtration |
| Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, 2021, 262, pp.117943. ⟨10.1016/j.carbpol.2021.117943⟩ |
| ISSN: | 0144-8617 |
| Popis: | International audience; Aspergillus spp. are well-known producers of pectinases commonly used in the industry. Aspergillus aculeatinus is a recently identified species but poorly characterized. This study aimed at giving a comprehensive characterization of the enzymatic potential of the O822 strain to produce Rhamnogalacturonan type I (RGI)-degrading enzymes. Proteomic analysis identified cell wall degrading enzymes (cellulases, hemicellulases, and pectinases) that accounted for 92 % of total secreted proteins. Twelve out of fifty proteins were identified as RGI-degrading enzymes. NMR and enzymatic assays revealed high levels of arabinofuranosidase, arabinanase, galactanase, rhamnogalacturonan hydrolases and rhamnogalacturonan acetylesterase activities in aqueous extracts. Viscosity assays carried out with RGI-rich camelina mucilage confirmed the efficiency of enzymes secreted by O822 to hydrolyze RGI, by decreasing viscosity by 70 %. Apple juice trials carried out at laboratory and pilot scale showed an increase in filtration flow rate and yield, paving the way for an industrial use of enzymes derived from A. aculeatinus. |
| Databáze: | OpenAIRE |
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