Altered vesicular glutamate transporter expression in human temporal lobe epilepsy with hippocampal sclerosis
| Autor: | Giulia Albertini, Cathy Jensen, Eleonora Aronica, Thomas Demuyser, Ellen Merckx, Eduard-Mihai Bentea, Ann Massie, Ilse Julia Smolders, Joeri Van Liefferinge |
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| Přispěvatelé: | ANS - Amsterdam Neuroscience, APH - Amsterdam Public Health, Pathology, Cellular and Computational Neuroscience (SILS, FNWI), Faculteit der Geneeskunde, Pharmaceutical and Pharmacological Sciences, Pathology/molecular and cellular medicine, Experimental Pharmacology, Neuro-Aging & Viro-Immunotherapy |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Messenger RNA
Pathology medicine.medical_specialty Hippocampal sclerosis Sclerosis General Neuroscience Glutamate receptor Hippocampus Hippocampal formation Biology medicine.disease Temporal lobe Epilepsy Real-time polymerase chain reaction Epilepsy Temporal Lobe Case-Control Studies Vesicular Glutamate Transport Protein 1 Vesicular Glutamate Transport Proteins Vesicular Glutamate Transport Protein 2 medicine Humans |
| Zdroj: | Neuroscience letters, 590, 184-188. Elsevier Ireland Ltd Neuroscience Letters, 590, 184-188. Elsevier |
| ISSN: | 0304-3940 |
| Popis: | Vesicular glutamate transporters (VGLUTs) are responsible for loading glutamate into synaptic vesicles. Altered VGLUT protein expression has been suggested to affect quantal size and glutamate release under both physiological and pathological conditions. In this study, we investigated mRNA and protein expression levels of the three VGLUT subtypes in hippocampal tissue of patients suffering from temporal lobe epilepsy (TLE) with hippocampal sclerosis (HS), International League Against Epilepsy type1 (ILAE typel) compared to autopsy controls, using quantitative polymerase chain reaction and semi-quantitative western blotting. mRNA expression levels of the VGLUTs are unaffected in hippocampal epileptic tissue compared to autopsy controls. At the protein level, VGLUT1 expression remains unaltered, while VGLUT2 is significantly decreased and VGLUT3 protein is significantly increased in hippocampal biopsies from TLE patients compared to controls. Our findings at the protein level can be explained by previously described histopathological changes observed in HS. Although VGLUTs have been repeatedly investigated in distinct rodent epilepsy models, their expression levels were hitherto not fully unraveled in the most difficult-to-treat form of epilepsy: TLE with HS ILAE type(We here, demonstrate for the first time that VGLUT2 protein expression is significantly decreased and VGLUT3 protein is significantly increased in the hippocampus of patients suffering from TLE with HS ILAE typel compared to autopsy controls. (c) 2015 Elsevier Ireland Ltd. All rights reserved |
| Databáze: | OpenAIRE |
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