DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product
| Autor: | Nullin Divecha, Stephen P. Jackson, Katharine O. Hartley, Arie Admon, Hong Zhang, Margery A. Connelly, Carl W. Anderson, Susan P. Lees-Miller, Graeme C. M. Smith, David A. Gell |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Complementary DNA repair DNA damage Molecular Sequence Data DNA-Activated Protein Kinase Protein Serine-Threonine Kinases Biology Phosphatidylinositol 3-Kinases General Biochemistry Genetics and Molecular Biology DNA-Dependent Protein Kinase Catalytic Subunit Ataxia Telangiectasia Humans Cloning Molecular Phosphorylation Protein kinase A DNA-PKcs Sequence Homology Amino Acid Biochemistry Genetics and Molecular Biology(all) Kinase Nuclear Proteins Proteins Lipid Metabolism Molecular biology DNA-Binding Proteins Phosphotransferases (Alcohol Group Acceptor) enzymes and coenzymes (carbohydrates) Ataxia telangiectasia and Rad3 related |
| Zdroj: | Cell. 82:849-856 |
| ISSN: | 0092-8674 |
| Popis: | DNA-dependent protein kinase (DNA-PK), which is involved in DNA double-stranded break repair and V(D)J recombination, comprises a DNA-targeting component called Ku and an ∼460 kDa catalytic subunit, DNA-PIK CS Here, we describe the cloning of the DNA-PK cs cDNA and show that DNA-PK cs falls into the phosphatidylinositol (PI) 3-kinase family. Biochemical assays, however, indicate that DNA-PK phosphorylates proteins but has no detectable activity toward lipids. Strikingly, DNA-PK cs is most similar to PI kinase family members involved in cell cycle control, DNA repair, and DNA damage responses. These include the FKBP12-rapamycin-binding proteins Tor1p, Tor2p, and FRAP, S. pombe rad3, and the product of the ataxia telangiectasia gene, mutations in which lead to genomic instability and predisposition to cancer. The relationship of these proteins to DNA-PK cs provides important clues to their mechanisms of action. |
| Databáze: | OpenAIRE |
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