Direct effects of apelin on cardiomyocyte contractility and electrophysiology
| Autor: | Klára Farkasfalvi, István Szokodi, Gopal K. Soppa, Mark A. Stagg, Magdi H. Yacoub, Nandor Marczin, Cesare M. Terracciano, Steven R. Coppen, Urszula Siedlecka, Joon Ha Lee |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Angiotensin receptor Myofilament Sodium-Hydrogen Exchangers Intracellular pH Biophysics Action Potentials Biochemistry Sarcomere Contractility Internal medicine medicine Animals Myocyte Myocytes Cardiac Molecular Biology Cells Cultured Dose-Response Relationship Drug Chemistry Cell Biology Myocardial Contraction Rats Apelin medicine.anatomical_structure Endocrinology Intercellular Signaling Peptides and Proteins Calcium Carrier Proteins Intercalated disc |
| Zdroj: | Biochemical and Biophysical Research Communications. 357:889-895 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.04.017 |
| Popis: | Apelin, the ligand for the angiotensin receptor like-1, has been implicated in the pathogenesis of atrial fibrillation and heart failure. However, it is unknown if apelin has direct effects on cardiomyocyte contractility and electrophysiology. APJ-like immunoreactivity was localized to T-tubules and intercalated disc area in isolated adult rat ventricular myocytes. Apelin (1 nM) significantly increased sarcomere shortening in normal as well as failing cardiomyocytes. The transient increase in shortening was not accompanied by increased [Ca 2+ ] transient amplitude. Apelin significantly activated the sarcolemmal Na + /H + exchanger (NHE) and increased intracellular pH. Moreover, apelin (10 nM) increased conduction velocity in monolayers of cultured neonatal rat cardiac myocytes. Our results demonstrate for the first time that apelin has direct effects on the propagation of action potential and contractility in cardiomyocytes. One of the mechanisms involved in the inotropic effect may be an increased myofilament sensitivity to Ca 2+ as apelin enhanced the activity of NHE with consequent intracellular alkalinization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect