Screening of microorganisms from A ntarctic surface water and cytotoxicity metabolites from A ntarctic microorganisms
| Autor: | Wei Wang, Jia Liu, Mengxin Xing, Mei Lv, Lanhong Zheng, Zhao Li, Yang Kangli, Jiancheng Zhu, Mi Sun, Daole Kang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Molecular mass biology Microorganism fermented active products microorganism screening MTT method 16S ribosomal RNA biology.organism_classification Antarctic surface water Microbiology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Biochemistry medicine cytotoxicity Cytotoxic T cell Fibroblast Cytotoxicity Antarctic microorganism Bacteria Original Research Food Science |
| Zdroj: | Food Science & Nutrition |
| ISSN: | 2048-7177 |
| DOI: | 10.1002/fsn3.273 |
| Popis: | The Antarctic is a potentially important library of microbial resources and new bioactive substances. In this study, microorganisms were isolated from surface water samples collected from different sites of the Antarctic. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay‐based cytotoxicity‐tracking method was used to identify Antarctic marine microorganism resources for antitumor lead compounds. The results showed that a total of 129 Antarctic microorganism strains were isolated. Twelve strains showed potent cytotoxic activities, among which a Gram‐negative, rod‐shaped bacterium, designated as N11‐8 was further studied. Phylogenetic analysis based on 16S rRNA gene sequence showed that N11‐8 belongs to the genus Bacillus. Fermented active products of N11‐8 with molecular weights of 1–30 kDa had higher inhibitory effects on different cancaer cells, such as BEL‐7402 human hepatocellular carcinoma cells, U251 human glioma cells, RKO human colon carcinoma cells, A549 human lung carcinoma cells, and MCF‐7 human breast carcinoma cells. However, they displayed lower cytotoxicity against HFL1 human normal fibroblast lung cells. However, they displayed lower cytotoxicity against HFL1 human normal fibroblast lung cells. Microscopic observations showed that the fermented active products have inhibitory activity on BEL‐7402 cells similar to that of mitomycin C. Further studies indicated that the fermented active products have high pH and high thermal stability. In conclusion, most strains isolated in this study may be developed as promising sources for the discovery of antitumor bioactive substances. The fermented active products of Antarctic marine Bacillus sp. N11‐ 8 are expected to be applied in the prevention and treatment of cancer. |
| Databáze: | OpenAIRE |
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