Airway dendritic cell maturation in children exposed to air pollution

Autor:	Frances R. Balkwill, Andrew J. Stagg, Rossa Brugha, Jonathan Grigg, Ameerah Khan, Harpal Kalsi, Benjamin Barratt, Naseem Mushtaq, Michele Padovan, Abigail Whitehouse, Lisa Miyashita, Lee Koh
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Male Sputum Cytology Physiology Social Sciences Families Spectrum Analysis Techniques Sociology Cellular types London Medicine Child Children Air Pollutants Schools Multidisciplinary medicine.diagnostic_test Immune cells Environmental exposure Flow Cytometry Pollution Body Fluids Spectrophotometry White blood cells Female Cytophotometry Anatomy medicine.symptom Research Article Spirometry Cell biology Blood cells Adolescent Science Immunology T cells Antigen-Presenting Cells Cytotoxic T cells Research and Analysis Methods Education Andrology Air Pollution Surface marker Humans Dc maturation Medicine and health sciences Biology and life sciences business.industry Ecology and Environmental Sciences Sputum Urban Health Dendritic Cells Environmental Exposure Dendritic cell Mucus Animal cells Age Groups People and Places Population Groupings Particulate Matter B7-2 Antigen business Airway
Zdroj:	PLoS ONE, Vol 15, Iss 5, p e0232040 (2020) PLoS ONE
ISSN:	1932-6203
Popis:	Urban particulate matter (PM) enhances airway dendritic cell (DC) maturation in vitro. However, to date, there are no data on the association between exposure to urban PM and DC maturation in vivo. We sought to determine whether exposure of school-age children (8 to 14 y) to PM was associated with expression of CD86, a marker of maturation of airway conventional DCs (cDC). Healthy London school children underwent spirometry and sputum induction. Flow cytometry was used to identify CD86 and CCR7 expression on cDC subsets (CD1c+ cDC2 and CD141+ cDC1). Tertiles of mean annual exposure to PM ≤ 10 microns (PM10) at the school address were determined using the London Air Quality Toolkit model. Tertiles of exposure from the 409 children from 19 schools recruited were; lower (23.1 to 25.6 μg/m3, n = 138), middle (25.6 to 26.8 μg/m3, n = 126), and upper (26.8 to 31.0 μg/m3, n = 145). DC expression was assessed in 164/370 (44%) children who completed sputum induction. The proportion (%) of cDC expressing CD86 in the lower exposure tertile (n = 47) was lower compared with the upper exposure tertile (n = 49); (52% (44 to 70%) vs 66% (51 to 82%), p
Databáze:	OpenAIRE
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