Durable HIV-1 antibody and T-cell responses elicited by an adjuvanted multi-protein recombinant vaccine in uninfected human volunteers
| Autor: | Helen Horton, Beryl A. Koblin, Guido Ferrari, Paul Spearman, William A. Blattner, M. Juliana McElrath, Gerald Voss, Mark Deers, Marguerite Koutsoukos, Sharon E. Frey, Louise Pedneault, Thomas G. Evans, Clayton Harro, Pierre Vandepapelière, Lindsey R. Baden, Georgia D. Tomaras, Paul A. Goepfert, Jonathan D. Fuchs, David C. Montefiori |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Adolescent medicine.medical_treatment T-Lymphocytes Enzyme-Linked Immunosorbent Assay CD8-Positive T-Lymphocytes HIV Antibodies HIV Envelope Protein gp120 Interferon-gamma Immune system Antigen Adjuvants Immunologic Double-Blind Method Neutralization Tests Malaria Vaccines medicine Humans HIV vaccine Neutralizing antibody Cell Proliferation AIDS Vaccines Immunity Cellular Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Middle Aged Virology Infectious Diseases Immunization Immunology Humoral immunity Antibody Formation biology.protein HIV-1 Molecular Medicine Female Antibody Adjuvant |
| Zdroj: | Vaccine. 25(3) |
| ISSN: | 0264-410X |
| Popis: | Background Use of the recombinant proteins NefTat and gp120 W61D formulated with the AS02A adjuvant system was previously shown to protect against AIDS in a rhesus macaque SHIV animal model system. Methods Eighty-four HIV uninfected human participants were vaccinated intramuscularly at 0, 1, and 3 months and evaluated for safety. Immune responses were analyzed for the presence of vaccine-induced antibody and T lymphocyte responses. Results The vaccines were safe and well tolerated at all doses. Nef-, Tat-, and gp120-specific binding antibodies were induced in all individuals that received the respective antigen, lasting up to 9 months after the final immunization. Antibodies able to neutralize the T-cell laboratory-adapted strain of HIV-1 W61D were detected in the majority of vacinees, but did not neutralize primary isolates. Envelope-specific antibody-dependent cell cytoxicity was detected in most of the individuals receiving gp120. Robust and persistent HIV-specific lymphoproliferative responses were detected against all subunit proteins in the majority of immunized participants. As expected, HIV-specific CD8 T-cell responses were not detected. Conclusions Despite the lack of primary isolate neutralizing antibody induction, the observed high frequency and magnitude of other immune responses warrant further work with this vaccine or vaccine components. |
| Databáze: | OpenAIRE |
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