Imaging of the bacterial interactions in lung co-infection in cystic fibrosis patients
| Autor: | A.V. Lazareva, O. V. Shaburova, Poddubikov Av, Maria V. Burkaltseva, N.A. Mikhailova, Zverev Vv, Victor N. Krylov, S.A. Lazarev, Sergey Krylov, E. A. Pleteneva |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Pathology medicine.medical_specialty bacterial interactions Epidemiology lcsh:QR1-502 alcaligenes faecalis Cystic fibrosis pseudomonas aeruginosa lcsh:Microbiology lcsh:Infectious and parasitic diseases cystic fibrosis 03 medical and health sciences 0302 clinical medicine co-infection Medicine Pharmacology (medical) lcsh:RC109-216 Lung business.industry medicine.disease 030104 developmental biology Infectious Diseases medicine.anatomical_structure 030228 respiratory system business Co infection |
| Zdroj: | Клиническая микробиология и антимикробная химиотерапия, Vol 22, Iss 2, Pp 155-160 (2020) |
| ISSN: | 2686-9586 1684-4386 |
| Popis: | Objective. To identify bacterial interactions at the site of infection in cystic fibrosis patients and to assess their possible effects on the course of infection. Materials and Methods. The following strains were used in this study: Alcaligenes faecalis LGBP strain, isolated from the environment; clinical isolates of Pseudomonas aeruginosa; Achromobacter xylosoxidans, Acinetobacter baumannii, Alcaligenes faecalis, and Bacillus subtilis strains; the standard laboratory P. aeruginosa PAO1 strain and its lysogens by temperate bacteriophages of various species, and its phageresistant mutants. Imaging and evaluation of the effects of bacterial interaction was performed in an in vitro co-infection with A. faecalis LGBP and the tested strains. Results. The bacteria of A. faecalis which are often involved in the lung co-infection in cystic fibrosis have been shown to stimulate the growth of most of the tested P. aeruginosa strains, as well as bacteria of some other species (for example, B. subtilis). The interspecies interactions pattern depends primarily on the strain of A. faecalis and physiological features of the infecting P. aeruginosa strains. When growing concurrently, the contacts between bacteria may change both the physical properties of the contacting bacteria surface (propagation rate) and the course of biochemical reactions in the contacting bacteria (occurrence of pigmentation, change in auto-plaquing pattern, reduction in alginate production). Conclusions. The results suggest that visually recognizable interactions are similar to the interactions of A. faecalis LGBP, exhibited in vitro with clinical isolates of P. aeruginosa, may influence on the course of chronic infections and their treatment results. Expanding of model studies of bacterial interspecies interactions may contribute to better understanding of their molecular mechanism that may be useful for optimizing therapy. |
| Databáze: | OpenAIRE |
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